Ethnopharmacological Relevance: Study of the interrelationships between human and the animals in their environment has always been a subject of interest and caused discoveries of the animal applications in medicine. From the latest century, these remedies called back in traditional medicine of Vietnam and South America and frog skin was used as a biological dressing and had good effects in healing wounds. Also, frog skin secretions have wound healing properties and reduce inflammation. In this study we applied these secretions in the form of ointment to investigate their healing activities.
Materials And Methods: Skin secretions were extracted from Rana ridibunda to evaluate their effects on wound healing in mice. Secretion used as raw extract (RE) and ultrafiltrated extract, using a membrane with cutoff 10kDa as under 10kDa (U10E), was administrated as ointment every 48h on wound site. Control group was left without any treatment and also there was other group treated with ointment (O group) alone. On 2, 4 and 6 days post injury, animals were euthanized and images were taken for wound closure evaluation. Then wound locations were removed for histological assays. Also wound microbial load was examined. Observational parameters including wound closure and wound microbiology in experimental groups compared with the control and O groups have been studied.
Results: The results showed U10E group has better effects than RE, O and control groups. Histological parameters, including numbers of inflammatory and fibroblast cells and amount of collagen fibers, neovascularization, as well, represented greater degree of wound healing in U10E group compared with RE, O, and control groups.
Conclusions: Our results showed that frog skin secretions were significantly effective in promoting wound healing process. The U10E extract from the frog R. ridibunda possesses a potent accelerating wound healing effect that promises good potential for clinical application in wound care. Further studies will be required to characterize special molecules encompassing healing properties.
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http://dx.doi.org/10.1016/j.jep.2012.12.016 | DOI Listing |
Discov Oncol
January 2025
Department of Anesthesiology, The First Affiliated Hospital, Zhejiang University School of Medicine, No.1367 Wenyi West Road, Yuhang District, Hangzhou, 311100, People's Republic of China.
The work is aimed to investigate whether midazolam functions in thyroid cancer and reveal the potential mechanism of action. Cell viability was detected by CCK-8 method when treated by varying doses of midazolam to detect the cytotoxicity of midazolam on human thyroid follicular epithelial cell line and thyroid cancer cell lines. In thyroid cancer cells, EDU staining, wound healing and transwell assays were respectively used to detect cell proliferation, migration and invasion.
View Article and Find Full Text PDFOdontology
January 2025
Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
We aimed to investigate the wound-healing, antioxidant, and anti-inflammatory effects of pterostilbene (PTS) on human gingival fibroblasts (GF). Different concentrations of PTS were applied to GFs and cell viability was evaluated by MTT assay. GFs were stimulated by lipopolysaccharide (LPS) and the study groups were determined as LPS, LPS + 1 μM PTS, LPS + 10 μM PTS, and control.
View Article and Find Full Text PDFTech Coloproctol
January 2025
Peritonectomy and Liver Cancer Unit, Department of Surgery, St George Hospital, Kogarah, NSW, Australia.
Background: The goal of cytoreductive surgery for peritoneal malignancy is to remove all macroscopic disease, which occasionally requires the excision of the umbilicus. While the absence of the umbilicus can be aesthetically undesirable for patients, umbilical reconstruction is rarely performed due to the perceived complexity and increased risk of wound infections (Sakata et al. in Colorectal Dis 23:1153-1157, 2021).
View Article and Find Full Text PDFLasers Med Sci
January 2025
Centro Universitário do Estado do Pará, Belém, Brazil.
The present study investigates the potential contribution of Photobiomodulation (PBM) to the regeneration of the bone following the extraction of the first mandibular molar in rats. The study evaluates the efficacy of PBM, using both Low-Level Laser Therapy (LLLT) and Light-Emitting Diode Therapy (LEDT), as promotors of osteoblastic activity and the formation of new bone. Study design, setting, and sample: 45 male Wistar rats were divided randomly into three groups of 15 individuals - (i) control group (left lower molar removed only), (ii) the LLL group (molar removed, followed by LLLT), and (iii) the LED group (molar removed, followed by LEDT).
View Article and Find Full Text PDFChem Biodivers
January 2025
UFES: Universidade Federal do Espirito Santo, Center of Exact, Natural and Health Sciences, Alto Universitário, Alegre, BRAZIL.
The sustainable use of pomegranate peel, a by-product of the food industry, is gaining importance in developing pharmaceutical bio-inputs, aligning with circular economy practices and waste reduction. This study explores the application of dry crude pomegranate peel extract (PPE) as a bio-input for medicinal gels with wound healing properties. PPE was extracted via percolation in ethanol and freeze-dried.
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