Many microRNAs reside in clusters in the genome, are generally similar in sequence, are transcribed in the same direction, and usually function synergistically. The miR-183/96/182 cluster is composed of 3 miRNA genes, and increased expression of miR-183, 96 and 182 are implicated in glioma carcinogenesis. Knockdown of individual components or of the entire miR-183/96/182 cluster inhibits the survival of glioma cells by regulating the ROS-induced apoptosis pathway. Furthermore, inhibition of the miR-183/96/182 cluster induced ROS-mediated AKT/survival independent of three target genes FGF9, CPEB1, and FOXO1, and inhibition of the miRNA cluster induced p53/apoptosis signaling, which was dependent on these same genes. In addition, knockdown of the miR-183/96/182 cluster enhanced the anticancer effect of Temozolomide on glioma cells by the ROS-mediated apoptosis pathway. Therefore, the miR-183/96/182 cluster may be a pleiotropic target for glioma therapy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2174/1568009611313020010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Circulating miRNA panels as a novel non-invasive diagnostic, prognostic, and potential predictive biomarkers in non-small cell lung cancer (NSCLC).
Br J Cancer
November 2024
Department of Medical Oncology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Background: Non-small cell lung cancer (NSCLC) is characterised by its aggressiveness and poor prognosis. Early detection and accurate prediction of therapeutic responses remain critical for improving patient outcomes. In the present study, we investigated the potential of circulating microRNA (miRNA) as non-invasive biomarkers in patients with NSCLC.
View Article and Find Full Text PDFThe miR-183/96/182 cluster regulates sensory innervation, resident myeloid cells and functions of the cornea through cell type-specific target genes.
Sci Rep
April 2024
Department of Ophthalmology, Visual and Anatomical Sciences, School of Medicine, Wayne State University, 540 E Canfield Street, Detroit, MI, 48201, USA.
The conserved miR-183/96/182 cluster (miR-183C) is expressed in both corneal resident myeloid cells (CRMCs) and sensory nerves (CSN) and modulates corneal immune/inflammatory responses. To uncover cell type-specific roles of miR-183C in CRMC and CSN and their contributions to corneal physiology, myeloid-specific miR-183C conditional knockout (MS-CKO), and sensory nerve-specific CKO (SNS-CKO) mice were produced and characterized in comparison to the conventional miR-183C KO. Immunofluorescence and confocal microscopy of flatmount corneas, corneal sensitivity, and tear volume assays were performed in young adult naïve mice; 3' RNA sequencing (Seq) and proteomics in the trigeminal ganglion (TG), cornea and CRMCs.
View Article and Find Full Text PDFpiRNAs in the human retina and retinal pigment epithelium reveal a potential role in intracellular trafficking and oxidative stress.
Mol Omics
May 2024
Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry-605014, India.
Long considered active only in the germline, the PIWI/piRNA pathway is now known to play a significant role in somatic cells, especially neurons. In this study, piRNAs were profiled in the human retina and retinal pigment epithelium (RPE). Furthermore, RNA immunoprecipitation with HIWI2 (PIWIL4) in ARPE19 cells yielded 261 piRNAs, and the expression of selective piRNAs in donor eyes was assessed by qRT-PCR.
View Article and Find Full Text PDFMicroRNA Expression Profile in Bone Marrow and Lymph Nodes in B-Cell Lymphomas.
Int J Mol Sci
October 2023
Department of the Structure and Function of Chromosomes, Laboratory of Molecular Genetics, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia.
Hodgkin's lymphomas (HL) and the majority of non-Hodgkin's lymphomas (NHL) derive from different stages of B-cell differentiation. MicroRNA (miRNA) expression profiles change during lymphopoiesis. Thus, miRNA expression analysis can be used as a reliable diagnostic tool to differentiate tumors.
View Article and Find Full Text PDFEssential role of the amino-terminal region of Drosha for the Microprocessor function.
iScience
October 2023
Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA 94143, USA.
Article Synopsis
- Drosha is a key player in processing primary microRNAs (pri-miRNAs) and regulating about 80 ribosomal protein genes, with mutations in its amino-terminal region linked to a vascular disorder.
- Researchers created a Drosha mutant (ΔN) lacking the amino-terminal region and found it couldn't process pri-miRNAs, leading to a significant depletion of most miRNAs, except for a specific cluster (miR-183/96/182).
- The study highlights that ΔN remains stable under nutrient lack, causing increased ribosomal protein production and protein synthesis, thus allowing cells to bypass growth arrest, emphasizing the importance of Drosha-NTR in miRNA production and nutrient-responsive control of translation.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!