In this study, we employed laser desorption/ionization mass spectrometry (LDI-MS) to explore the interactions between thiolated DNA (HS-DNA) and gold nanoparticles (Au NPs). Under nanosecond-pulsed laser irradiation (Nd:YAG, 355 nm), the efficiency of Au cluster ion formation from the Au NPs decreased in the presence of HS-DNA. At the optimal laser power density (2.1 × 10(4) W cm(-2)), the intensity of the Au cluster signal was sensitive to the DNA coverage and the length of the DNA strands on the Au NPs (diameter: 13 nm). Using this information, we developed a simple and specific DNA sensor that operates through analysis of the Au cluster ions formed from the fragmentation of Au NPs under LDI conditions. The coverage of the thiolated probe DNA (pDNA) on the Au NPs increased in the presence of its perfectly matched DNA (DNA(pm)). As a result, the intensity of the signal of Au cluster ions decreased upon increasing the concentration of DNA(pm). Coupling these pDNA-Au NPs with LDI-MS allowed the detection of DNA(pm) at concentrations down to the nanomolar regime. Furthermore, we applied this pDNA-Au NP probe to the detection of single-nucleotide polymorphisms (SNPs) of the Arg249Ser unit in the TP53 gene. To the best of our knowledge, this paper provides the first example of the use of LDI to analyze the coverage and structure of DNA strands on metal NPs. This simple, rapid, high-throughput detection system, based on the coupling of biofunctional Au NPs with LDI-MS, appears to hold great practicality for bioanalyses of oligonucleotides and proteins.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ac302847n | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Single Rare-Earth Ion Doped Tin-Oxo Nanocluster Photoresists for High-Resolution Extreme Ultraviolet Lithography.
Nano Lett
January 2025
Institute of Modern Optics, College of Electronic Information and Optical Engineering, Nankai University, Tianjin 300350, China.
Rare-earth (RE) metals are known as industrial vitamins and show significant regulatory effects in many fields. In this work, we first demonstrated that the vitamin effect of RE metals can also be applied to extreme ultraviolet (EUV) lithography. Using a SnRE oxo cluster as the universal platform, different individual RE metal ions were successfully doped to obtain a series of isomorphic heterometallic clusters (RE = Y, Sm, Eu, Ho, Er).
View Article and Find Full Text PDFCuproplasia and cuproptosis, two sides of the coin.
Cancer Commun (Lond)
January 2025
Department of Medical Oncology, Zhejiang Key Laboratory of Multi-omics Precision Diagnosis and Treatment of Liver Diseases, Cancer Center of Zhejiang University, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, P. R. China.
Copper is an essential micronutrient in the human body, mainly acting as a crucial cofactor required for a wide range of physiological processes across nearly all cell types. Recent advances revealed that tumor cells seize copper to fulfill their rapid proliferation, metastasis, immune evasion, and so on by reprogramming the copper regulatory network, defined as cuproplasia. Thus, targeting copper chelation to reduce copper levels has been considered a rational tumor therapy strategy.
View Article and Find Full Text PDFFluorescence-enhanced detection of sulfide ions through tuning the structure-activity relationship of gold nanoclusters.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
Hebei Key Lab of Power Plant Flue Gas Multi-Pollutants Control, Department of Environmental Science and Engineering, North China Electric Power University, Baoding 071003, China; Guangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China. Electronic address:
The concentration of S is a vital environmental indicator for evaluating the quality of source water, surface water, and wastewater, and it has a significant impact on biological systems, particularly human health. Therefore, it is crucial to detect S selectively and sensitively. In this study, we developed a simple and rapid one-pot method to prepare a gold nanocluster (BSA-AuNCs) probe for fluorescence-enhanced detection of S toxemia and analyzed the morphological characteristics of BSA-AuNCs and its complex with S using various characterization techniques.
View Article and Find Full Text PDFA Novel Nitrite Reductase from for Efficient Degradation of Nitrite.
Biomolecules
January 2025
Xingzhi College, Zhejiang Normal University, Jinhua 321100, China.
Nitrite reductases play a crucial role in the nitrogen cycle, demonstrating significant potential for applications in the food industry and environmental remediation, particularly for nitrite degradation and detection. In this study, we identified a novel nitrite reductase (NiR) from a newly isolated denitrifying bacterium, YD01. We constructed a heterologous expression system using BL21/pET28a-Nir, which exhibited remarkable nitrite reductase enzyme activity of 29 U/mL in the culture broth, substantially higher than that reported for other strains.
View Article and Find Full Text PDFOptimization of dried garlic physicochemical properties using a self-organizing map and the development of an artificial intelligence prediction model.
Sci Rep
January 2025
Agricultural Engineering Department, Faculty of Agriculture and Natural Resources, Aswan University, Aswan, Egypt.
The experiments were conducted at different levels of infrared power, airflow, and temperature. The relationships between the input process factors and response factors' physicochemical properties of dried garlic were optimized by a self-organizing map (SOM), and the model was developed using machine learning. Artificial Neural Network (ANN) with 99% predicting accuracy and Self-Organizing Maps (SOM) with 97% clustering accuracy were used to determine the quality characteristics of garlic.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!