Detection of foot-and-mouth disease serotype O by ELISA using a monoclonal antibody.

Hybridoma (Larchmt)

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu, P.R. China.

Published: December 2012

An ELISA assay with monoclonal antibody (MELISA) was used to type serotype O of foot-and-mouth disease virus (FMDV). All FMDV serotype O reference strains were positive by MELISA, while other viruses such as FMDV serotypes Asia 1, C, A and classical swine fever virus, swine vesicular disease virus, and porcine reproductive and respiratory syndrome virus remained negative. Further, FMDV serotype O positive samples were able to be detected by MELISA. This assay may be particularly suitable for diagnosis of FMDV serotype O infection in field stations.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3526892PMC
http://dx.doi.org/10.1089/hyb.2012.0081DOI Listing

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