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Single-Cell Transcriptomics Identifies Selective Lineage-Specific Regulation of Genes in Aortic Smooth Muscle Cells in Mice.
Arterioscler Thromb Vasc Biol
January 2025
Division of Cardiology, Department of Medicine, University of Washington (S.S., S.J., N.S., C.Y.L., L.L., D.A.D.).
Background: Smooth muscle cells (SMCs) of the proximal thoracic aorta are derived from second heart field (SHF) and cardiac neural crest lineages. Recent studies, both in vitro and in vivo, have implied relevance of lineage-specific SMC functions in the pathophysiology of thoracic aortic diseases; however, whether 2 lineage-derived SMCs have any predisposed transcriptional differences in the control aorta remains unexplored.
Methods: Single-cell RNA sequencing and single-nucleus assay for transposase-accessible chromatin sequencing were performed on isolated cells from the aortic root and ascending aortas of 14-week-old SHF-traced () and cardiac neural crest-traced () male mice.
NGF-releasing Prussian blue nanoparticles for nerve injury repair of lumbar disc herniation.
Front Chem
December 2024
Department of Algology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Introduction: Compression of the nerve root by a lumbar disc herniation can cause radiating pain in the lower limbs, and the nerve root decompression treatment may leave some patients with motor dysfunction and reduced sensory function. Studies have shown that nerve growth factor (NGF) can promote nerve growth and repair, but high doses, long duration, and immune response have become bottlenecks of its clinical application.
Methods: To overcome this obstacle, we developed Prussian blue (PBs) nanoparticles with the bio-delivery function and antioxidant effects of nanoenzymes.
Morpho-physiological and transcriptomic responses of field pennycress to waterlogging.
Front Plant Sci
December 2024
Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH, United States.
Field pennycress () is a new biofuel winter annual crop with extreme cold hardiness and a short life cycle, enabling off-season integration into corn and soybean rotations across the U.S. Midwest.
View Article and Find Full Text PDFAnalysis of root number and canal morphology of maxillary premolars using cone-beam computed tomography.
Imaging Sci Dent
December 2024
Department of Oral and Maxillofacial Radiology, School of Dentistry, Pusan National University, Yangsan, Korea.
Purpose: This study aimed to evaluate the number of roots and type of root canals in maxillary first and second premolars within a selected Korean population utilizing cone-beam computed tomography (CBCT). Additionally, it sought to investigate potential differences in these features according to sex and tooth type.
Materials And Methods: CBCT images of 585 maxillary first premolars and 578 maxillary second premolars from 303 patients were retrospectively reviewed.
Genome-wide identification, evolution and expression analysis unveil the role of genes in nitrogen utilization and nitrogen allocation.
Physiol Mol Biol Plants
December 2024
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010 Sichuan Province China.
Unlabelled: The rapid growth of Bamboo made the uptake and allocation of nitrogen much important. Nitrate is the main form that plant utilized nitrogen by nitrate transporters (NRTs) as well as ammonium salt. In this study, we identified 155 genes which mapped to 32 chromosomes out of 35 chromosomes in .
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