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Recombinant high density lipoprotein nanoparticles for target-specific delivery of siRNA. | LitMetric

Recombinant high density lipoprotein nanoparticles for target-specific delivery of siRNA.

Pharm Res

School of Pharmacy, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, 200240, People's Republic of China.

Published: May 2013

AI Article Synopsis

  • Researchers developed a novel recombinant high density lipoprotein (rHDL) vector designed to efficiently deliver small interfering RNA (siRNA) for gene regulation aimed at treating diseases.
  • The rHDL nanoparticles showed high siRNA encapsulation efficiency and effectively silenced gene expression in liver cancer cells with minimal toxicity.
  • The study suggests that these rHDL nanoparticles could be a promising method for targeted siRNA delivery, particularly to the liver, leveraging natural uptake pathways.

Article Abstract

Purpose: Regulation of gene expression using small interfering RNA (siRNA) is a promising strategy for treatments of numerous diseases. However, the progress towards broad application of siRNA requires the development of safe and effective vectors that target to specific cells. In this study, we developed a novel recombinant high density lipoprotein (rHDL) vector with high siRNA encapsulation efficiency.

Methods: They were prepared by condensing siRNA with various commercial cationic polymers and coating the polyplex with a layer of lipids and apolipoprotein AI (apo AI). The rHDL nanoparticles were used to transfect SMMC-7721 hepatoma cells with stable luciferase expression. The uptake and intracellular trafficing of siRNA were also investigated.

Results: Characterization studies revealed these rHDL nanoparticles had similar physical properties as natural HDLs. The various rHDL formulations had high silencing efficiency (more than 70% knockdown) in hepatocytes with minimum cytotoxicity. Moreover, the uptake of rHDL by SMMC-7721 was confirmed to be mediated through the natural HDL uptake pathway.

Conclusions: The work described here demonstrated the optimized rHDL nanoparticles may offer a promising tool for siRNA delivery to the liver.

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Source
http://dx.doi.org/10.1007/s11095-012-0957-4DOI Listing

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