Quantification of IncP-1 plasmid prevalence in environmental samples.

Appl Environ Microbiol

Julius Kühn-Institut-Federal Research Center for Cultivated Plants, Department of Epidemiology and Pathogen Diagnostics, Braunschweig, Germany.

Published: February 2013

To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568613PMC
http://dx.doi.org/10.1128/AEM.03728-12DOI Listing

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