To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3568613 | PMC |
http://dx.doi.org/10.1128/AEM.03728-12 | DOI Listing |
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