A combination approach for rapid and high yielding purification of bacterial minicells.

J Microbiol Methods

Department of Pharmacology and Toxicology, B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Sarkhej-Gandhinagar Highway, Thaltej, Ahmedabad, 380 054 Gujarat, India.

Published: March 2013

AI Article Synopsis

  • A new method for purifying bacterial minicells combines antibiotic lysis with filtration.
  • This process is quick, inexpensive, and produces a high yield of purified minicells.
  • The method ensures no contamination from the parent strain, as confirmed by fluorescent microscopy and particle size analysis.

Article Abstract

A method for bacterial minicell purification was developed by combining antibiotic (ceftriaxone) lysis and filtration. This method is fast, cost effective and facilitates high yield of purified minicells, with no parent strain contamination as confirmed by fluorescent microscopy, average particle size and polydispersity index.

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http://dx.doi.org/10.1016/j.mimet.2012.12.002DOI Listing

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