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Robust single-nucleus RNA sequencing reveals depot-specific cell population dynamics in adipose tissue remodeling during obesity.
Elife
January 2025
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, United States.
Single-nucleus RNA sequencing (snRNA-seq), an alternative to single-cell RNA sequencing (scRNA-seq), encounters technical challenges in obtaining high-quality nuclei and RNA, persistently hindering its applications. Here, we present a robust technique for isolating nuclei across various tissue types, remarkably enhancing snRNA-seq data quality. Employing this approach, we comprehensively characterize the depot-dependent cellular dynamics of various cell types underlying mouse adipose tissue remodeling during obesity.
View Article and Find Full Text PDFIndoor Air Quality in Multi-Family Housing: Drivers and Interventions.
Curr Environ Health Rep
January 2025
Brandeis University, Waltham, MA, USA.
Purpose Of Review: Indoor air pollution is likely to be elevated in multi-family housing and to contribute to health disparities, but limited studies to date have systematically considered the empirical evidence for exposure differentials between multi-family and single-family housing. Our goal is to separately examine the drivers of residential indoor air pollution, including outdoor air pollution, ventilation and filtration, indoor sources, and occupant activity patterns, using secondhand smoke as a case study to examine the behavioral dimensions of indoor environmental interventions.
Recent Findings: Within studies published from 2018 to 2023, multi-family homes have higher average outdoor air pollution than single-family homes given their more frequent presence in urban and near-roadway settings.
Cost-effectiveness analysis of PD-L1 testing associated with pembrolizumab for first-line treatment of advanced non-small cell lung cancer in China.
Int J Clin Pharm
January 2025
Center for Health Policy and Technology Evaluation, Peking University Health Science Center, Beijing, 100191, China.
Background: Lung cancer is the leading cause of cancer-related deaths in China, and pembrolizumab shows differential efficacy in advanced non-small cell lung cancer (NSCLC) with different PD-L1 expression levels.
Aim: To assess the cost-effectiveness of PD-L1 testing associated with pembrolizumab for first-line treatment of NSCLC from the perspective of Chinese healthcare system.
Method: Over a lifetime horizon, a three-state partitioned survival model was developed to assess the cost-effectiveness of PD-L1 testing and no PD-L1 testing.
Mental Health Consequences of Opioid Pain Medication Use Behaviors and Motives Among Adolescents and Young Adults With Cancer: Results From a National Survey.
Psychooncology
January 2025
Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, Washington, USA.
Background: Adolescents and young adults (AYA) with cancer experience long-term consequences into survivorship that impact quality of life, including mental health symptoms, substance use, and persistent pain. Given the elevated rates of pain, AYA cancer survivors are at increased risk for opioid pain medication (OPM) exposure, increasing risk for opioid-related negative consequences, particularly for those with mental health symptoms. Minimal research has documented that a considerable proportion of AYAs with cancer receive OPM that continues into survivorship, yet the lack of consensus on the definition of problematic opioid use coupled with the high clinical need for OPM makes it particularly challenging to understand the impact of OPM use in this population.
View Article and Find Full Text PDFis an important spice and medicinal plant widely utilized in East and Southeast Asia. Non-targeted metabolomics techniques were employed to study the variations in the content and composition of essential oil from during drying at different temperatures: 40°C, 50°C, 60°C, and 70°C. A total of 260 metabolites were detected using gas chromatography-mass spectrometry (GC-MS), mainly terpenoids and aldehydes.
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