An electrochemical hybridization assay involving neutravidin-coated carbon screen-printed electrodes and an HRP-based detection have been shown to provide an effective tool for the genotypic analysis of extended-spectrum β-lactamase-producing E. coli strains in complex samples such as soil.
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http://dx.doi.org/10.1016/j.mimet.2012.11.019 | DOI Listing |
Microbiology (Reading)
January 2025
Instituto de Microbiologa, Colegio de Ciencias Biolgicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador.
Antimicrobial resistance (AMR) is a major threat to global public health that continues to grow owing to selective pressure caused by the use and overuse of antimicrobial drugs. Resistance spread by plasmids is of special concern, as they can mediate a wide distribution of AMR genes, including those encoding extended-spectrum -lactamases (ESBLs). The CTX-M family of ESBLs has rapidly spread worldwide, playing a large role in the declining effectiveness of third-generation cephalosporins.
View Article and Find Full Text PDFJ Water Health
January 2025
Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac 34000, Serbia.
Extended spectrum -lactamase (ESBL)-producing Enterobacteriaceae, including and , pose a serious risk to human health because of antibiotic resistance. Wastewater serves as a reservoir for these bacteria, contributing to the evolution and transmission of antibiotic-resistant strains. The research aims to identify ESBL bacterium in wastewater samples from District Kohat.
View Article and Find Full Text PDFJ Microbiol Biol Educ
January 2025
Department of Microbiology, University of Georgia, Athens, Georgia, USA.
We present a laboratory module that uses isolation of antibiotic-resistant bacteria from locally collected stream water samples to introduce undergraduate students to basic microbiological culture-based and molecular techniques. This module also educates them on the global public health threat of antibiotic-resistant organisms. Through eight laboratory sessions, students are involved in quality testing of water sources from their neighborhoods, followed by isolation of extended-spectrum beta-lactamase-producing .
View Article and Find Full Text PDF<b>Background and Objective:</b> It is well documented that Whole Genome Sequencing (WGS) has recently used to explore new resistance patterns and track the dissemination of extensive and pan drug-resistant microbes in healthcare settings. This article explores the link between traumatic infections caused by road traffic accidents (RTAs) leading to coma and the development of chest infections caused by extensively drug-resistant (XDR) <i>Klebsiella pneumoniae</i> and <i>Pseudomonas aeruginosa</i>. <b>Materials and Methods:</b> The study was carried out from March to December 2022 which included a 45-year-old male patient admitted to the ICU of Al Ramadi Teaching Hospitals following a severe RTA that resulted in a TBI and subsequent coma.
View Article and Find Full Text PDFHolographic displays have the potential to reconstruct natural light field information, making them highly promising for applications in augmented reality (AR), head-up displays (HUD), and new types of transparent three-dimensional (3D) displays. However, current spatial light modulators (SLMs) are constrained by pixel size and resolution, limiting display size. Additionally, existing holographic displays have narrow viewing angles due to device diffraction limits, algorithms, and optical configurations.
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