A disposable immunosensor based on electrochemically reduced graphene oxide (ERGO) was developed for the detection of Enterobacter sakazakii. First, the graphene was deposited on a screen-printed carbon electrode (SPCE) by an electrochemical method. Second, the horseradish peroxidase-labeled bacteria-specific antibody was assembled onto the modified electrode to enhance the sensitivity of the immunosensor. The immunosensor constructed in this study can sensitively and rapidly detect E. sakazakii, and this method may contribute to further foodborne pathogen detection. In addition, this ERGO-modified SPCE could also provide new insights into the development of immunosensors for other bacteria to facilitate rapid detection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ab.2012.11.013 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Highly Sensitive Creatine Kinase Detection in Human Serum using 11-mercaptoundecanoic acid Modified ITO-PET Electrodes.
Anal Biochem
January 2025
Çanakkale Onsekiz Mart University, Faculty of Engineering, Bioengineering Department, Çanakkale-TURKEY. Electronic address:
The enzyme creatine kinase (CK) is a biomarker that plays an extremely significant role in the early detection of cardiovascular disorders. Serum levels of CK are regularly monitored in patients with heart attacks, one of the most critical cardiovascular illnesses. In this study, a highly sensitive electrochemical immunosensor system was designed for the importance of early diagnosis of CK.
View Article and Find Full Text PDFDisposable and sensitive electrochemical magneto-immunosensor for point-of-care HCV diagnostics: Targeting HCVcAg, the active viremia biomarker, in patient samples.
Biosens Bioelectron
December 2024
Centre for Biomedicine, Hull York Medical School, University of Hull, Hull HU6 7RX, United Kingdom. Electronic address:
Early detection of hepatitis C virus (HCV) infection is crucial for eliminating this silent killer, especially in resource-limited settings. HCV core antigen (HCVcAg) represents a promising alternative to the current "gold standard" HCV RNA assays as an active viremia biomarker. Herein, a highly sensitive electrochemical magneto-immunosensor for the HCVcAg was developed.
View Article and Find Full Text PDFLeveraging self-signal amplifying poly(acrylic acid)/polyaniline electrodes for label-free electrochemical immunoassays in protein biomarker detection.
Bioelectrochemistry
December 2024
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address:
Accurate quantification of specific biomarkers is essential for clinical diagnosis and evaluating therapeutic efficacy. A self-signal-amplifying poly(acrylic acid) (PAA)/polyaniline (PANI) film-modified disposable and cost-effective screen-printed carbon electrode (SPCE) has been developed for constructing new label-free immunosensors targeting two model biomarkers: human immunoglobulin G (IgG) and alpha-fetoprotein (AFP). The electrochemically deposited PAA/PANI film on the SPCE serves a dual function: both a bio-immobilization support and a signal amplifier, enhancing biomarker detection sensitivity and efficiency.
View Article and Find Full Text PDFPoint-of-Care Detection of Carcinoembryonic Antigen (CEA) Using a Smartphone-Based, Label-Free Electrochemical Immunosensor with Multilayer CuONPs/CNTs/GO on a Disposable Screen-Printed Electrode.
Biosensors (Basel)
December 2024
Research Laboratory for Analytical Instrument and Electrochemistry Innovation, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
In order to identify carcinoembryonic antigen (CEA) in serum samples, an innovative smartphone-based, label-free electrochemical immunosensor was created without the need for additional labels or markers. This technology presents a viable method for on-site cancer diagnostics. The novel smartphone-integrated, label-free immunosensing platform was constructed by nanostructured materials that utilize the layer-by-layer (LBL) assembly technique, allowing for meticulous control over the interface.
View Article and Find Full Text PDFDisposable and eco-friendly electrochemical immunosensor for rapid detection of SARS-CoV-2.
Talanta
October 2023
Instituto de Química, Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil; Instituto Nacional de Ciência e Tecnologia de Bioanalítica, 13084-971, Campinas, SP, Brazil. Electronic address:
This study describes the development of a simple, disposable, and eco-friendly electrochemical immunosensor for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Electrochemical devices were manufactured by stencil-printing using low-cost materials such as polyester sheets, graphite flakes, and natural resin. The immunosensor comprises gold nanoparticles stabilized with cysteamine, glutaraldehyde, anti-SARS-CoV-2 S protein monoclonal antibody (Ab1) as the biological receptor, and bovine serum albumin as a protective layer.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!