For improving the performance and sludge settling property of an activated sludge reduction process with uncoupler, in this investigation, uncoupler and nano-sized magnetic particles were added simultaneously to a sequencing batch reactor for exploring their synergistic effects to the characteristics of activated sludge. The results showed that the volume reduction of sludge reached 41% with single 2,4,5-Trichlorophenol (TCP) Comparing with the control experiment, the biodegradability and settling properties of the activated sludge decreased. Under the actions of TCP combined with nano-sized magnetic particles, the volume reduction of sludge reached 34%, the removal efficiencies of COD, nitrogen, and phosphorus as well as the sludge settling property were not significantly influenced. After 31 d's operation, the dehydrogenase activity was improved by 10%-18% and exhibited an accumulative effect over time. It was observed with an optical microscope that the species and amounts of protozoon and metazoan increased and a compact structure of sludge floc was formed. The results also indicated that using nano-sized magnetic particles and uncoupler could restrict the yield of excess sludge and improve the performance of an activated sludge system.

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