Premise Of The Study: Microsatellite makers were developed in Kleinia neriifolia, an endemic Asteraceae species on the Canary Islands, for investigation of the population genetic structure and colonization history of this insular species. •
Methods And Results: Seventeen polymorphic and seven monomorphic microsatellite loci were isolated and characterized from K. neriifolia using the protocol of Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO). At the population level, the number of alleles detected per locus varied from two to 16, and the observed and expected heterozygosities were 0.105-1.000 and 0.100-0.923, respectively. •
Conclusions: These highly informative loci are potentially useful to obtain a deeper understanding of the evolutionary process of K. neriifolia.
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http://dx.doi.org/10.3732/ajb.1200166 | DOI Listing |
Mol Ecol
November 2024
Department of Biodiversity and Conservation, Real Jardín Botánico (RJB), CSIC, Madrid, Spain.
Multiple-island endemics (MIE) are considered ideal natural subjects to study patterns of island colonization that involve recent population-level genetic processes. Kleinia neriifolia is a Canarian MIE widespread across the archipelago, which exhibits a close phylogenetic relationship with species in northwest Africa and at the other side of the Sahara Desert. Here, we used target sequencing with plastid skimming (Hyb-Seq), a dense population-level sampling of K.
View Article and Find Full Text PDFAnn Bot
February 2019
Departamento de Biodiversidad Molecular y Banco de ADN, Jardín Botánico Canario 'Viera y Clavijo' - Unidad Asociada CSIC, Cabildo de Gran Canaria, Camino del Palmeral 15 de Tafira Alta, Las Palmas de Gran Canaria, Spain.
Background And Aims: Archipelagos provide a valuable framework for investigating phenotypic evolution under different levels of geographical isolation. Here, we analysed two co-distributed, widespread plant lineages to examine if incipient island differentiation follows parallel patterns of variation in traits related to dispersal and colonization.
Methods: Twenty-one populations of two anemochorous Canarian endemics, Kleinia neriifolia and Periploca laevigata, were sampled to represent mainland congeners and two contrasting exposures across all the main islands.
Front Plant Sci
June 2017
Escuela Nacional de Ciencias Biológicas-Instituto Politécnico NacionalMexico City, Mexico.
Haw. is an endemic species on the Canarian archipelago, this species is widespread in the coastal thicket of all the Canarian islands. In the present study, genetic diversity and population structure of were investigated using chloroplast gene sequences and nuclear SSR (simple sequence repeat).
View Article and Find Full Text PDFAm J Bot
December 2012
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
Premise Of The Study: Microsatellite makers were developed in Kleinia neriifolia, an endemic Asteraceae species on the Canary Islands, for investigation of the population genetic structure and colonization history of this insular species. •
Methods And Results: Seventeen polymorphic and seven monomorphic microsatellite loci were isolated and characterized from K. neriifolia using the protocol of Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO).
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