Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.
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| http://dx.doi.org/10.3390/ijms131114243 | DOI Listing |
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Article Synopsis
- Climate change affects the ability of forest tree species to adapt, particularly in terms of drought resilience; understanding the genetics behind this is crucial for conservation efforts.
- New research focused on the fir species Abies pinsapo, exposed to controlled drought conditions, revealed significant gene expression differences between resilient and sensitive tree phenotypes during and after drought.
- Findings included the identification of specific genetic markers (SNPs) connected to drought resilience, offering insights that could inform strategies to conserve this vulnerable species.
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