AI Article Synopsis
- Two monoclonal antibodies (BrBm37 and BrBm38) were developed to target the triosephosphate isomerase (RmTIM) enzyme in the Rhipicephalus (Boophilus) microplus tick.
- BrBm37 and BrBm38 effectively inhibited RmTIM activity by up to 85% and 98%, respectively, depending on the concentration of the antibodies used.
- Treatment with these antibodies also reduced RmTIM activity in various tick tissues and inhibited the growth of embryonic tick cells.
Article Abstract
In the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition by BrBm37 was up to 85% whereas that of BrBrm38 was 98%, depending on the antibody-enzyme ratio. RmTIM activity was lower in ovarian, gut, and fat body tissue extracts treated with BrBm37 or BrBm38 mAbs. The proliferation of the embryonic tick cell line (BME26) was inhibited by BrBm37 and BrBm38 mAbs. In summary, the results reveal that it is possible to interfere with the RmTIM function using antibodies, even in intact cells.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3497315 | PMC |
| http://dx.doi.org/10.3390/ijms131013118 | DOI Listing |
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Inhibition of enzyme activity of Rhipicephalus (Boophilus) microplus triosephosphate isomerase and BME26 cell growth by monoclonal antibodies.
Int J Mol Sci
October 2012
Laboratory of Biochemistry Hatisaburo Masuda, Institute of Medical Biochemistry, Federal University of Rio de Janeiro, NUPEM - UFRJ/Macaé, Av. São José do Barreto 764, São José do Barreto, Macaé, RJ, CEP 27971-550, Brazil.
Article Synopsis
- Two monoclonal antibodies (BrBm37 and BrBm38) were developed to target the triosephosphate isomerase (RmTIM) enzyme in the Rhipicephalus (Boophilus) microplus tick.
- BrBm37 and BrBm38 effectively inhibited RmTIM activity by up to 85% and 98%, respectively, depending on the concentration of the antibodies used.
- Treatment with these antibodies also reduced RmTIM activity in various tick tissues and inhibited the growth of embryonic tick cells.
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