This paper proposes a spectral imaging technology by synchronizing a programmable light source and a high-speed monochrome camera. The light source is capable of emitting arbitrary spectrum in high speed, so that the system has the advantage of capturing spectral images without using filters. The camera and the light source are controlled by a computer in order to capture image sequence synchronously with camera and illuminant control signals. First, we describe a projector for spectrally rendering a real scene as a fundamental usage of the spectral imaging system. Second, we describe the effective applications to (1) spectral reflectance recovery and (2) tristimulus imager. The performances of the proposed algorithms to solve the applied problems are examined in experiments in detail. We demonstrate potential applicability of the proposed spectral imaging technology.
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http://dx.doi.org/10.1364/JOSAA.29.001764 | DOI Listing |
J Neurochem
January 2025
Core Facility Small Animal MRI, Ulm University, Ulm, Germany.
Proton magnetic resonance spectroscopy (MRS) offers a non-invasive, repeatable, and reproducible method for in vivo metabolite profiling of the brain and other tissues. However, metabolite fingerprinting by MRS requires high signal-to-noise ratios for accurate metabolite quantification, which has traditionally been limited to large volumes of interest, compromising spatial fidelity. In this study, we introduce a new optimized pipeline that combines LASER MRS acquisition at 11.
View Article and Find Full Text PDFJ Biophotonics
January 2025
Department of Electronic Engineering, Maynooth University, Kildare, Ireland.
Broadband CARS is a coherent Raman scattering technique that provides access to the full biological vibrational spectrum within milliseconds, facilitating the recording of widefield hyperspectral Raman images. In this work, BCARS hyperspectral images of unstained cells from two different cell lines of immune lineage (T cell [Jurkat] and pDCs [CAL-1]) were recorded and analyzed using multivariate statistical algorithms in order to determine the spectral differences between the cells. A classifier was trained which could distinguish the known cells with a 97% out-of-bag accuracy.
View Article and Find Full Text PDFAnal Methods
November 2017
Centre de Recherche sur la Conservation (CRC), MNHN, Sorbonne-Universités CNRS, MCC, USR 3224, CP21, 36 rue Geoffroy Saint Hilaire, 75005 Paris, France.
Reflectance spectral imaging is a powerful tool for the non-invasive study of cultural heritage objects. Particular visible to short wave infrared (400-2500 nm) spectral features are linked to compositional information. Spectral images can hence be used to generate useful chemical maps.
View Article and Find Full Text PDFBMC Ophthalmol
January 2025
Department of Ophthalmology, Linkou main branch, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Background: While vaccination remains crucial in mitigating the impact of the COVID-19 pandemic, several ocular adverse events has been reported, including Acute Zonal Occult Outer Retinopathy (AZOOR) complex.
Case Presentation: A 31-year-old female presented declined best corrected visual acuity (BCVA) and flashes in both eyes three days following second recombinant mRNA COVID-19 vaccine (Moderna). Fundus autofluorescence (FAF) illustrated speckled hyper-AF lesions surrounding right eye torpedo maculopathy site and hyper-AF lesions in the left macula.
BMC Ophthalmol
January 2025
Department of Ophthalmology, Medical Faculty, University Hospital of Cologne, Kerpener Strasse 62, 50937, Cologne, Germany.
Background/ Aims: To analyze the longitudinal change in Bruch's membrane opening minimal rim width (BMO-MRW) and peripapillary retinal nerve fiber layer (pRNFL) thickness using optical coherence tomography (OCT) after implantation of a PRESERFLO® microshunt for surgical glaucoma management in adult glaucoma patients.
Methods: Retrospective data analysis of 59 eyes of 59 participants undergoing implantation of a PRESERFLO microshunt between 2019 and 2022 at a tertiary center for glaucoma management. Surgical management included primary temporary occlusion of the glaucoma shunt to prevent early hypotony.
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