AI Article Synopsis
- * The study focuses on the role of the enzyme Gld2, which may help stabilize the miRNA miR-122 by adding a single adenine nucleotide to its 3' end, although the exact cause-and-effect relationship is unclear.
- * Researchers found that Gld2 directly adds adenine to specific miRNA types in human fibroblasts, which enhances their stability, and this stabilization depends on the nucleotide composition at the miRNA's 3' end, contributing to our understanding of gene regulation processes.
Article Abstract
MicroRNAs (miRNAs) are small, noncoding RNAs that inhibit translation and promote mRNA decay. The levels of mature miRNAs are the result of different rates of transcription, processing, and turnover. The noncanonical polymerase Gld2 has been implicated in the stabilization of miR-122, possibly through catalyzing 3' monoadenylation; however, there is little evidence that this relationship is one of cause and effect. Here, we biochemically characterize Gld2's involvement in miRNA monoadenylation and its effect on miRNA stability. We find that Gld2 directly monoadenylates and stabilizes specific miRNA populations in human fibroblasts and that sensitivity to monoadenylation-induced stability depends on nucleotides in the miRNA 3' end. These results establish a mechanism of miRNA stability and resulting posttranscriptional gene regulation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3534910 | PMC |
| http://dx.doi.org/10.1016/j.celrep.2012.10.023 | DOI Listing |
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