Premise Of The Study: Microsatellite markers were isolated and characterized in Mimulus ringens (Phrymaceae), a herbaceous wetland perennial, to facilitate studies of mating patterns and population genetic structure. •
Methods And Results: A total of 42 polymorphic loci were identified from a sample of 24 individuals from a single population in Ohio, USA. The number of alleles per locus ranged from two to nine, and median observed heterozygosity was 0.435. •
Conclusions: This large number of polymorphic loci will enable researchers to quantify male fitness, patterns of multiple paternity, selfing, and biparental inbreeding in large natural populations of this species. These markers will also permit detailed study of fine-scale patterns of genetic structure.
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http://dx.doi.org/10.3732/ajb.1200180 | DOI Listing |
Am J Bot
July 2024
Department of Biological Sciences, University of Wisconsin, Milwaukee, 53211, WI, USA.
Premise: Cross-fertilization in most flowering plants is facilitated by mobile animals that transport pollen while foraging for floral rewards. The contributions of different visitors can vary widely, depending on the amount of pollen transferred during a single visit and on the frequency and timing of the visits of each pollinator taxon.
Methods: We used three approaches to measure the pollination value of bees that visit Mimulus ringens: pollinator interviews, field population observations, and caging studies.
Am J Bot
January 2024
Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin, 53201, USA.
Premise: The timing and pattern of a plant's flowering can have important consequences for reproductive success. Variation in flowering phenology may influence the number of prospective mates, the risk of mating with lower quality individuals, and the likelihood of self-pollination. Here we use a common garden experiment to explore within- and among-population variation in phenology.
View Article and Find Full Text PDFJ Evol Biol
November 2021
Department of Biological Sciences, Binghamton University (SUNY), Binghamton, New York, USA.
Seasonal timing traits are commonly under recurrent, spatially variable selection, and are therefore predicted to exhibit clinal variation. Temperate perennial plants often require vernalization to prompt growth and reproduction; however, little is known about whether vernalization requirements change across the range of a broadly distributed species. We performed a critical vernalization duration study in Mimulus ringens, coupled with population genomic analysis.
View Article and Find Full Text PDFJ Evol Biol
May 2021
Department of Biology, University of Akron, Akron, OH, USA.
Variation in selfing rates within and among populations of hermaphroditic flowering plants can strongly influence the evolution of reproductive strategies and the genetic structure of populations. This intraspecific variation in mating patterns may reflect both genetic and ecological factors, but the relative importance of these factors remains poorly understood. Here, we explore how selfing in 13 natural populations of the perennial wildflower Mimulus ringens is influenced by (a) pollinator visitation, an ecological factor, and (b) floral display, a trait with a genetic component that also responds to environmental variation.
View Article and Find Full Text PDFAoB Plants
August 2020
Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Researchers have long assumed that plant spatial location influences plant reproductive success and pollinator foraging behaviour. For example, many flowering plant populations have small, linear or irregular shapes that increase the proportion of plants on the edge, which may reduce mating opportunities through both male and female function. Additionally, plants that rely on pollinators may be particularly vulnerable to edge effects if those pollinators exhibit restricted foraging and pollen carryover is limited.
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