The uptake of liposomal contents into Catharanthus roseus protoplasts has been quantitated. Hi.phest uptake was obtained from positively charged vesicles (1.09% of vesicle contents per 10(7) protoplasts) in the presence of 10% w/v polyethylene glycol 4000. Significant uptake was also observed from negatively charged vesicles (0.1% of vesicle contents per 10(7) protoplasts) in the presence of 25% polyethylene glycol 1540. The uptake of vesicle contents from negatively charged liposomes was confirmed by light microscopy after treatment of the protoplasts with liposomes loaded with the fluorescent dye 6-carboxyfluorescein. About 10% of the treated protoplasts exhibited intracellular fluorescence. No uptake from neutral liposomes was detected.
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