Enzymatic Synthesis of t,2-di-Sinapoylglucose from 1-Sinapoylglucose by a Protein Preparation from Cotyledons of Raphanus sstivus Grown in the Dark.

Protein preparation from cotyledons of Raphanus sativus seedlings grown in the dark catalyzed the transfer of the sinapoyl moiety of 1-O-sinapoyl-ß-D-glucose to the C-2 hydroxyl group of the glucose moiety of another molecule of 1-O-sinapcyl-ß-D-glucose to form 1,2-di-O-sinapoyl-ß-D-glucopyranose. In this synthesis the 1-O-acyl glucoside was used both as acyl donor and acceptor molecule. Maximal rate of product formation was found to be at pH 7.5 in 0.1M Hepes buffer.