AI Article Synopsis
- Protoplasts from Helianthus tuberosus tubers were prepared in different developmental stages, and vacuoles were isolated and purified.
- All fructan (inulin) with a degree of polymerization (DP) of 3 or more was found only in the vacuoles, while other sugars were only partially present there.
- The vacuoles also contained key enzymes for synthesizing and degrading fructan, highlighting their role as essential organelles for fructan storage and metabolism.
Article Abstract
Protoplasts were prepared from tubers of Helianthus tuberosus in the developing and in the resting stage of development. Vacuoles were isolated from the protoplasts and purified by sedimentation through a density gradient of glycine betaine. All the fructan (inulin) with a DP ⩾ 3 (i.e. the trisaccharide isokestose and larger polymers of fructose) was found to be located exclusively in the vacuoles whilst sucrose, glucose, and fructose were located only partially there. The vacuoles were also found to be the sole cell compartments containing fructan synthesizing enzyme activities (sucrose-sucrose-fructosyl-transferase and fructan-fructan-fructosyl-transferase) and fructan degrading enzyme activity (fructan exohydrolase), depending on the stage of development of the tubers. The vacuole is therefore proposed to be the cell organelle for fructan storage and metabolism.
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| http://dx.doi.org/10.1016/S0176-1617(84)80089-9 | DOI Listing |
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