AI Article Synopsis
- MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression by binding to specific mRNA regions, affecting protein production.
- A study identified that miRNA-965 and miRNA-1926 can potentially target the Fibroin L chain (Fib-L) mRNA, suggesting they play a role in controlling its expression.
- Experimental tests involving luciferase assays demonstrate that these miRNAs can lower Fib-L expression by interacting with its 3' untranslated region.
Article Abstract
MicroRNAs (miRNAs) are an abundant class of approximately 22-nucleotide (nt)-short noncoding RNA molecules present in the genomes of all multicellular organisms that act through base pairing to partially complementary sequences of the 3'untranslated region (UTR) of targeted mRNAs. Using bioinformatic approach, we found that the 3'UTR of the Fibroin L chain (Fib-L) mRNA matches perfectly the nucleotides 2-8 at the 5' end of the miRNA-965 and miRNA-1926. These two miRNAs might act as regulators of Fib-L gene expression at the post-transcriptional level. To examine whether Fib-L is directly targeted by miRNA-965 and miRNA-1926 in vitro, miRNA expression vectors and target reporter vector with 3'UTR of Fib-L were constructed respectively. Two vectors were co-transfected into Sf21cells. The luciferase assay showed that miRNA-965 and miRNA-1926 may down regulate the expression of Fib-L via complementary interaction with the target sites in 3'UTR.
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| http://dx.doi.org/10.1134/s1068162012030168 | DOI Listing |
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