We propose a direct and real-time displacement measurement using an optical frequency comb, able to compensate optically for index of refraction variations due to atmospheric parameters. This scheme could be useful for applications requiring stringent precision over a long distance in air, a situation where dispersion becomes the main limitation. The key ingredient is the use of a mode-locked laser as a precise source for multi-wavelength interferometry in a homodyne detection scheme. By shaping temporally the local oscillator, one can directly access the desired parameter (distance variation) while being insensitive to fluctuations induced by parameters of the environment such as pressure, temperature, humidity and CO2 content.
Department of Bioengineering, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, Cancer Center at Illinois, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
High-resolution optical microscopy, particularly super-resolution localization microscopy, requires precise real-time drift correction to maintain constant focus at nanoscale precision during the prolonged data acquisition. Existing methods, such as fiducial marker tracking, reflection monitoring, and bright-field image correlation, each provide certain advantages but are limited in their broad applicability. In this work, a versatile and robust drift correction technique is presented for single-molecule localization-based super-resolution microscopy.
Invasive meningococcal disease, caused by (), is a critical global health issue, necessitating swift and precise diagnostics for effective management and control. Here, we introduce a novel diagnostic assay, NM-RT-MCDA, that combines multiple cross displacement amplification (MCDA) with real-time fluorescence detection, targeting a specific gene region in the genome. The assay utilizes a primer set designed for high specificity and incorporates a fluorophore-quencher pair with a restriction endonuclease site for real-time monitoring.
A major challenge in epigenetics is uncovering the dynamic distribution of nucleosomes and other DNA-binding proteins, which plays a crucial role in regulating cellular functions. Established approaches such as ATAC-seq, ChIP-seq, and CUT&RUN provide valuable insights but are limited by the ensemble nature of their data, masking the cellular and molecular heterogeneity that is often functionally significant. Recently, long-read sequencing technologies, particularly Single Molecule, Real-Time (SMRT/PacBio) sequencing, have introduced transformative capabilities, such as N6-methyladenine (6mA) footprinting.
Compared to quantitative real-time PCR (q-PCR), CRISPR-Cas-mediated technology is more suitable for point-of-care testing (POCT) and has potential for wider application in the future. Generally, the operational procedure of CRISPR-Cas-mediated diagnostic method consists of two independent steps, the reaction of signal amplification and the CRISPR-Cas-mediated signal detection. Complex multi-step procedures can easily lead to cross-contamination.
State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, Nanjing 210023, China.
Real-time visualization of messenger RNA (mRNA) is essential for tumor classification, grading, and staging. However, the low signal-to-background ratios and nonspatiotemporal specific signal amplification restricted the in vivo imaging of mRNA. In this study, a near-infrared (NIR) light-activated DNA nanodevice (DND) was developed for spatiotemporal in vivo fluorescence imaging of mRNA.
