AI Article Synopsis
- New generation HPLC columns feature superficially porous silica particles from manufacturers like Halo and Kinetex, offering ultra-fast separations with moderate sample loading and low back pressure.
- These columns utilize various chemistries, including C(8), C(18), and hydrophilic interaction, with silica particles typically measuring 2.7 and 1.7 μm in diameter.
- The article discusses the columns' textures, chemistries, optimization, comparisons, and future developments in the field.
Article Abstract
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate sample loading capacity and low back pressure. Chemistries of these columns are C(8), C(18), RP-Amide, hydrophilic interaction liquid chromatography, penta fluorophenyl (PFP), F5, and RP-aqua. Normally, the silica gel particles are of 2.7 and 1.7 μm as total and inner solid core diameters, respectively, with 0.5-μm-thick of outer porous layer having 90 Å pore sizes and 150 m(2)/g surface area. This article describes these new generation columns with special emphasis on their textures and chemistries, separations, optimization, and comparison (inter and intra stationary phases). Besides, future perspectives have also been discussed.
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| http://dx.doi.org/10.1002/jssc.201200454 | DOI Listing |
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