Loop-mediated isothermal amplification (LAMP) method was discovered in the last decade but only used for the first time in the diagnosis of mink enteritis virus (MEV) infection in this study. The amplification could be completed within 60 min, under isothermal condition at 65°C, by employing a set of four primers targeting the VP2 gene of MEV. The LAMP was more sensitive than the conventional PCR, with a detection limit of 10(-1) median tissue culture infective doses (TCID(50))/ml per reaction, compared with 10 TCID(50)/ml for PCR analysis. No cross reactivity was observed for other related viruses, including canine distemper virus (CDV) and Aleutian mink disease parvovirus (AMDV). Eighty four of 230 clinical samples were found to be positive for MEV, which is higher than that determined by using the conventional PCR method (68). The results indicate the LAMP can be potentially used to determine MEV as a simple, rapid procedure. This assay would be an available alternative to PCR analysis for the diagnosis of MEV infection in mink, particularly in less well-equipped laboratories and in rural settings where resources are limited.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jviromet.2012.11.012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multiplex one-step RT‒qPCR assays for simultaneous detection of AMDV, MEV and CDV.
BMC Vet Res
January 2025
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Background: Aleutian mink disease, mink viral enteritis and canine distemper are known as the three most serious diseases that cause great economic loss in the mink industry. In clinical practice, aleutian mink disease virus (AMDV), mink enteritis virus (MEV) and canine distemper virus (CDV) are common mixed infections, and they have similar clinical clinical signs, such as diarrhoea. Therefore, a rapid and accurate differential diagnosis method for use on mink ranches is essential for the control of these three pathogens.
View Article and Find Full Text PDFMink enteritis virus infection induced cell cycle arrest and autophagy for its replication.
Vet Microbiol
January 2025
Shandong Provincial Key Laboratory of Zoonoses, Shandong Agricultural University, Taian, Shandong Province 271018, China; College of Veterinary Medicine, Shandong Agricultural University, Taian, Shandong Province 271018, China. Electronic address:
Mink enteritis virus (MEV) is an important pathogen causing mink viral enteritis. The mechanisms of cell cycle arrest induced by MEV infection and the roles of autophagy in MEV replication remain unclear. In this study, the roles of MEV NS1 protein in inducing cell cycle arrest were investigated, using the in vitro CRFK cell models.
View Article and Find Full Text PDFTwo novel sites determine genetic relationships between CPV-2 and FPV: an epidemiological survey of canine and feline parvoviruses in Changchun, China (2020).
Front Vet Sci
November 2024
Department of Viral Infectious Diseases of Special Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China.
Canine parvovirus (CPV-2) and feline parvovirus (FPV) cause severe hemorrhagic diarrhea disease in dogs, cats, and fur-bearing and wildlife carnivores worldwide, continuing to pose significant threats. In this study, 140 rectal swabs were collected from 70 domestic dogs and 70 cats with clinical diarrhea in veterinary clinics in Changchun during 2020. A total of 64.
View Article and Find Full Text PDFDevelopment and characterization of a novel specific monoclonal antibody against mink enteritis virus and its antigen epitope analysis.
Microb Pathog
July 2024
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, China. Electronic address:
This study prepared a novel monoclonal antibody (MAb) against mink enteritis parvovirus (MEV) and identified its antigen epitope. The antibody subclass is identified as IgG1, the titers of the MAb is up to 1:1 × 10 and keeps stably after low-temperature storage for 9 months or 11 passages of the MAb cells. The MAb can specifically recognize MEV in the cells in IFA, but not Aleutian disease virus (ADV) or canine distemper virus (CDV).
View Article and Find Full Text PDFEstablishment and application of multiplex PCR for rapid detection of three mink diarrhea-associated viruses.
J Virol Methods
July 2024
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, PR China. Electronic address:
Article Synopsis
- - A multiplex PCR method was created to detect three viruses that cause diarrhea in mink: circovirus (MCV), bocavirus (MBoV), and enteritis virus (MEV) by using specially designed primers for each virus.
- - This optimized PCR successfully amplified specific DNA fragments for each virus and proved to be ten times more sensitive than traditional singleplex PCR while avoiding cross-reactivity with other relevant pathogens.
- - The study tested mink fecal samples from China and found occurrences of the three viruses, with results aligning perfectly with singleplex PCR, demonstrating the method's effectiveness for rapid virus detection and epidemiological studies.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!