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The acid tolerant and cold-active β-galactosidase from Lactococcus lactis strain is an attractive biocatalyst for lactose hydrolysis. | LitMetric

The acid tolerant and cold-active β-galactosidase from Lactococcus lactis strain is an attractive biocatalyst for lactose hydrolysis.

Antonie Van Leeuwenhoek

Laboratory for Biocrystallography and Structural Biology of Therapeutic Targets, Bases Moléculaires des Systèmes Infectieux-UMR 5086, CNRS/Université de Lyon 1, Institut de Biologie et Chimie des Protéines-FR3302, 7 Passage du Vercors, 69367, Lyon Cedex 07, France.

Published: April 2013

AI Article Synopsis

Article Abstract

The gene encoding the β-galactosidase from the dairy Lactococcus lactis IL1403 strain was cloned, sequenced and overexpressed in Escherichia coli. The purified enzyme has a tetrameric arrangement composed of four identical 120 kDa subunits. Biochemical characterization showed that it is optimally active within a wide range of temperatures from 15 to 55 °C and of pH from 6.0 to 7.5. For its maximal activity this enzyme requires only 0.8 mM Fe(2+) and 1.6 mM Mg(2+). Purified protein displayed a high catalytic efficiency of 102 s(-1) mM(-1) for lactose. The enzyme stability was increased by immobilization mainly at low pH (from 4.0 to 5.5) and high temperatures (55 and 60 °C). The bioconversion of lactose using the L. lactis β-galactosidase allows the production of lactose with a high bioconversion rate (98 %) within a wide range of pH and temperature.

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Source
http://dx.doi.org/10.1007/s10482-012-9852-6DOI Listing

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