AI Article Synopsis
- The study investigates the differences in excitatory synaptic proteins between the striatum and hippocampus, finding lower levels of certain proteins in striatal extracts.
- While CaMKII interacts more strongly with spinophilin in the striatum, the types of protein complexes formed vary between synaptic and extrasynaptic locations in both brain regions.
- These selective differences in protein interactions and assembly may influence how excitatory transmission is regulated differently in the two areas of the brain.
Article Abstract
Distinct physiological stimuli are required for bidirectional synaptic plasticity in striatum and hippocampus, but differences in the underlying signaling mechanisms are poorly understood. We have begun to compare levels and interactions of key excitatory synaptic proteins in whole extracts and subcellular fractions isolated from micro-dissected striatum and hippocampus. Levels of multiple glutamate receptor subunits, calcium/calmodulin-dependent protein kinase II (CaMKII), a highly abundant serine/threonine kinase, and spinophilin, a F-actin and protein phosphatase 1 (PP1) binding protein, were significantly lower in striatal extracts, as well as in synaptic and/or extrasynaptic fractions, compared with similar hippocampal extracts/fractions. However, CaMKII interactions with spinophilin were more robust in striatum compared with hippocampus, and this enhanced association was restricted to the extrasynaptic fraction. NMDAR GluN2B subunits associate with both spinophilin and CaMKII, but spinophilin-GluN2B complexes were enriched in extrasynaptic fractions whereas CaMKII-GluN2B complexes were enriched in synaptic fractions. Notably, the association of GluN2B with both CaMKII and spinophilin was more robust in striatal extrasynaptic fractions compared with hippocampal extrasynaptic fractions. Selective differences in the assembly of synaptic and extrasynaptic signaling complexes may contribute to differential physiological regulation of excitatory transmission in striatum and hippocampus.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3557548 | PMC |
| http://dx.doi.org/10.1111/jnc.12101 | DOI Listing |
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