To develop a specific, rapid, and convenient immunochromatography assay (ICA) to detect melamine residues in dairy products and feed sample. Colloidal gold particles labeled with purified monoclonal antibody against anti-melamine were used as the detector reagent. The MEL-OVA (the conjugate of melamine and ovalbumin) and goat anti-mouse melamine IgG were blotted on the test and control regions of nitrocellulose membrane. The strip was then assembled with sample pad, absorbing pad, and dorsal shield. The limit of detection (LOD) is 50 microg/L. The test trip was applied to detect melamine in milk, milk powder, and animal feeds, with detection limits of 100 microg/L for milk, 100 ng/g for milk powder, 200 ng/g for feeds. Compared to LC-MS/MS, the ICA could be used to screen a large number of dairy products and feed samples for melamine residue.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Bionic Luminescent Sensors Based on Covalent Organic Frameworks: Auditory, Gustatory, and Olfactory Information Monitoring for Multimode Perception.
ACS Nano
January 2025
Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Siping Road 1239, Shanghai 200092, China.
The synthesis of covalent organic frameworks (COFs) with excellent luminescent properties and their effective application in the field of bionic sensing remain a formidable challenge. Herein, a series of COFs with different numbers of hydroxyl groups are successfully synthesized, and the number of hydroxyl groups on the benzene-1,3,5-tricarbaldehyde (BTA) linker influences the properties of the final COFs. The COF (HHBTA-OH) prepared with hydrazine hydrate (HH) and BTA containing one hydroxyl group as the ligands exhibits the best fluorescent performance.
View Article and Find Full Text PDFNitrogen@Carbon Quantum Dots for Fluorescence Detection of L-alanine, L-methionine and L-cysteine.
J Fluoresc
January 2025
School of Materials and Chemical Engineering, West Anhui University, Lu'an, Anhui, 237012, China.
Nitrogen@Carbon quantum dots (N@CQDs) are prepared using microwave hydrothermal method, and polyvinylpyrrolidone (PVP) and melamine are used as mixed C source and N source. Microwave reaction conditions of preparing the N@CQDs are 170 ℃ and 3 h. This N@CQDs are are used as fluorescence probe for detection of amino acids.
View Article and Find Full Text PDFPortable SpectroChip-Based Immunoassay Platform for Rapid and Accurate Melamine Quantification in Urine Samples.
Toxics
November 2024
Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Growing concerns about the health risks of melamine adulteration in food products highlight the urgent need for reliable detection methods. However, the long-term effects of chronic low-level melamine exposure remain inadequately explored. This study introduces THE ONE InstantCare platform, a portable immunoassay analyzer integrating a SpectroChip-based spectral processing unit (SPU) with lateral flow immunoassay (LFIA) for sensitive and accurate quantification of melamine in human urine.
View Article and Find Full Text PDFA review of chitosan role in milk bioactive-based drug delivery, smart packaging and biosensors: Recent advances and developments.
Int J Biol Macromol
December 2024
Nanotechnology Department, School of Advanced Technologies, Iran University of Science and Technology (IUST), Tehran, Iran. Electronic address:
Chitosan, a versatile biopolymer derived from chitin, is increasingly recognized in the milk industry for its multifunctional applications in drug delivery, smart packaging, and biosensor development. This review provides a comprehensive analysis of recent advances in chitosan production techniques. These include chemical, biological, and novel methods such as deep eutectic solvents (DES), microwave-assisted approaches, and laser-assisted processes.
View Article and Find Full Text PDFAssociations of prenatal urinary melamine, melamine analogues, and aromatic amines with gestational duration and fetal growth in the ECHO Cohort.
Environ Int
December 2024
Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, CB#7435, Chapel Hill, NC 27599-7435, United States. Electronic address:
Melamine, its analogues, and aromatic amines (AAs) were commonly detected in a previous study of pregnant women in the Environmental influences on Child Health Outcomes (ECHO) Cohort. While these chemicals have identified toxicities, little is known about their influences on fetal development. We measured these chemicals in gestational urine samples in 3 ECHO cohort sites to assess associations with birth outcomes (n = 1,231).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!