Background: The influenza virus is responsible for causing major respiratory tract symptoms. A fast, accurate diagnosis is essential for efficient treatment, especially in patients with complications. The Rapid Influenza Diagnostic Tests (RIDTs) for influenza detection have been developed to subtype the influenza virus. The re-evaluation of the rapid test is needed in terms of specificity, sensitivity, and accuracy.
Methods: From August 13, 2010 to September 22, 2011, 1,076 nasal aspirates were obtained from patients, ages ranging from 15 days to 98 years, with symptoms of influenza-like illness (ILI) and evaluated by 2 types of RIDTs, Standard Diagnosis (SD) and QuickVue (QV) Rapid tests followed by real-time RT-PCR. The results from the rapid test diagnoses were compared to those from real-time RT-PCR.
Results: During 2010 and 2011, the estimated sensitivity of the SD rapid test for seasonal H3, human pandemic H1N1, and influenza B infection was 49.4%, specificity was 84.1%, positive predictive value was 47.6%, and negative predictive value was 85% while those of the QV rapid test were 63.4%, 96.7%, 94.8%, and 80.3%, respectively. Infant patients (< or = 5 years) yielded less false negatives while adolescents and adults (older than 5 years) showed more false negatives 8.8% and 15.2%, respectively. Using rapid test diagnosis, H3N2 influenza virus was found with more false negative results (11.1%) than the other viruses (1.1 - 3.5%). The SD rapid test appeared to be more sensitive than the QV test during high season activity while the QV test was more sensitive during the period of low influenza virus activity.
Conclusions: Due to persistent genetic drift of the influenza virus, the available RIDTs should be re-evaluated each year. During 2010 - 2011, the QV rapid test showed more reliable results than the SD rapid test. However, the false negative results of H3N2 influenza virus detection during its peak should be cause for concern. Some of the results, e.g. patients with complications, should be compared with real-time RT-PCR as the gold standard method for detecting influenza virus infection.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Characteristics of viral ovarian tumor domain protease from two emerging orthonairoviruses and identification of Yezo virus human infections in northeastern China as early as 2012.
J Virol
December 2024
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, China.
Emerging tick-borne orthonairovirus infections pose a growing global concern, with limited understanding of the viral ovarian tumor-like cysteine proteases (vOTUs) encoded by novel orthonairoviruses. These vOTUs, a group of deubiquinylases (DUBs), disrupt the innate immune response. Yezo virus (YEZV), a recently discovered pathogenic orthonairovirus, was first reported in Japan in 2021.
View Article and Find Full Text PDFInfluenza B virus infection alters the regenerative potential of murine alveolar type 2 pneumocytes.
mBio
December 2024
Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, North Carolina, USA.
Unlabelled: Respiratory epithelial cells can survive direct infection by influenza viruses, and the long-term consequences of that infection have been characterized in a subset of proximal airway cell types. The impact on the cells that survive viral infection in the distal lung epithelia, however, is much less well-characterized. Utilizing a Cre-expressing influenza B virus (IBV) and a lox-stop-lox tdTomato reporter mouse model, we identified that alveolar type 2 (AT2) pneumocytes, a progenitor cell type in the distal lung, can survive viral infection.
View Article and Find Full Text PDFThe current situation with H5N1 highly pathogenic avian influenza virus (HPAI) is causing a worldwide concern due to multiple outbreaks in wild birds, poultry, and mammals. Moreover, multiple zoonotic infections in humans have been reported. Importantly, HPAI H5N1 viruses with genetic markers of adaptation to mammals have been detected.
View Article and Find Full Text PDFSimultaneous Detection of Five Infectious Diseases in a Single Strip: Oligo dT-Utilized Lateral Flow Immunoassay.
Anal Chem
January 2025
SB BIOSCIENCE Inc., Room 120, Venture Building, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
The need for accurate and simultaneous diagnosis of multiple respiratory infectious diseases has become increasingly critical due to ongoing viral mutations and the similarity of symptoms among various viruses. Here, we have advanced our detection capabilities by developing a multiplex lateral flow immunoassay (LFA) platform that integrates oligonucleotides and antibodies, enabling the simultaneous detection of five respiratory viruses: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Influenza A (FluA), Influenza B (FluB), Respiratory syncytial virus (RSV), and Adenovirus (ADV), on a single membrane. By applying the oligonucleotide and antibody-conjugated AuNPs, the platform enables highly sensitive and specific detection.
View Article and Find Full Text PDFHighly Pathogenic Avian Influenza (HPAI) H5N1 virus in Finland in 2021-2023 - Genetic diversity of the viruses and infection kinetics in human dendritic cells.
Emerg Microbes Infect
January 2025
Microbiology Unit, Finnish Institute for Health and Welfare, Helsinki, Finland.
Highly pathogenic avian influenza (HPAI) H5N1 is known for its virulence and zoonotic potential, infecting birds and mammals, thus raising public health concerns. Since 2021 its spread among birds has led to cross-species transmission causing epizootics among mammals, eventually impacting fur animal farms in Finland in 2023. To analyze the infectivity of the Finnish H5N1 isolates in human cells, representatives of diverse H5N1 isolates were selected based on the genetic differences, host animal species, and the year of occurrence.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!