[Liver phospholipids composition and activity of glutathione redox-system in rats on prolonged high-fat diet].

We studied liver phospholipid composition and activity of the glutathione redox-system in rats on prolonged for 180 days high-fat diet. The adaptive response was formed from day 30 to day 90 of high-fat load and was associated with increased relative content of phosphatidylseryne and phosphatidylethanolamine homeostasis. Constant antioxidant activity of glutathione redox-system for 30 days period of high-fat load was achieved by stable glutathioneperoxidase activity, on 90th day--level of glutathione reductase increased. The detected fluctuations in phospholipid composition and activity of glutathione redox-system in liver tissue on 30-90th days of the experiment indicated the formation of a compensatory response to maintain cell integrity. On 180th day of high-fat load due to exhaustion of compensatory mechanisms in the glutathione antioxidant defense and intensification of lipid peroxidation the lipid matrix of cytomembrane underwent profound restructuring associated with decreased levels of phosphatidylinositol, phosphatidylethanolamine, increased proportion of apoptotic phospholipids (phosphatidylserine, sphingomyelin). Such phospholipids composition on the background of glutathione redox-system inhibition can determine apoptosis, necrosis and liver fibrogenesis. These results extend the knowledge about mechanisms of liver adaptation and disadaptation to nutritional stress.