Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00277-012-1621-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Structure-function analysis of carrier protein-dependent 2-sulfamoylacetyl transferase in the biosynthesis of altemicidin.
Nat Commun
December 2024
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
The general control non-repressible 5 (GCN5)-related N-acetyltransferase (GNAT) SbzI, in the biosynthesis of the sulfonamide antibiotic altemicidin, catalyzes the transfer of the 2-sulfamoylacetyl (2-SA) moiety onto 6-azatetrahydroindane dinucleotide. While most GNAT superfamily utilize acyl-coenzyme A (acyl-CoA) as substrates, SbzI recognizes a carrier-protein (CP)-tethered 2-SA substrate. Moreover, SbzI is the only naturally occurring enzyme that catalyzes the direct incorporation of sulfonamide, a valuable pharmacophore in medicinal chemistry.
View Article and Find Full Text PDFMinimal presynaptic protein machinery governing diverse kinetics of calcium-evoked neurotransmitter release.
Nat Commun
December 2024
Nanobiology Institute, Yale University, West Haven, CT, USA.
Neurotransmitters are released from synaptic vesicles with remarkable precision in response to presynaptic calcium influx but exhibit significant heterogeneity in exocytosis timing and efficacy based on the recent history of activity. This heterogeneity is critical for information transfer in the brain, yet its molecular basis remains poorly understood. Here, we employ a biochemically-defined fusion assay under physiologically relevant conditions to delineate the minimal protein machinery sufficient to account for various modes of calcium-triggered vesicle fusion dynamics.
View Article and Find Full Text PDFThe structural basis of protective and nonprotective human monoclonal antibodies targeting the parainfluenza virus type 3 hemagglutinin-neuraminidase.
Nat Commun
December 2024
Center for Vaccines and Immunology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Parainfluenza virus 3 (PIV3) infection poses a substantial risk to vulnerable groups including infants, the elderly, and immunocompromised individuals, and lacks effective treatments or vaccines. This study focuses on targeting the hemagglutinin-neuraminidase (HN) protein, a structural glycoprotein of PIV3 critical for viral infection and egress. With the objective of targeting these activities of HN, we identified eight neutralizing human monoclonal antibodies (mAbs) with potent effects on viral neutralization, cell-cell fusion inhibition, and complement deposition.
View Article and Find Full Text PDFBridged emulsion gels from polymer-nanoparticle enabling large-amount biomedical encapsulation and functionalization.
Nat Commun
December 2024
Key Laboratory of Materials Chemistry for Energy Conversion and Storage of Ministry of Education (HUST), School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, 430074, China.
Large-amount encapsulation and subsequent expressing are common characteristics for many biomedical applications, such as cosmetic creams and medical ointments. Emulsion gels can accomplish that, but often undergo exclusive, complex, multiple synthesis steps, showing extremely laborious and non-universal. The method here is simple via precisely interfacial engineering in homogenizing a nanoparticle aqueous dispersion and a polymer oil solution, gaining interfacial 45° three-phase-contact-angle for the nanoparticle that can bridge across oil emulsions' interfaces and ultimately form interconnected macroscopic networks.
View Article and Find Full Text PDFCholesky Decomposition and the Second-Derivative Two-Electron Integrals Required for the Computation of Magnetizabilities Using Gauge-Including Atomic Orbitals.
J Phys Chem A
December 2024
Department Chemie, Johannes Gutenberg-Universität Mainz, Duesbergweg 10-14, D-55128 Mainz, Germany.
The computation of magnetizability tensors using gauge-including atomic orbitals is discussed in the context of Cholesky decomposition (CD) for the two-electron repulsion integrals with a focus on the involved doubly differentiated integrals. Three schemes for their handling are suggested: the first exploits the density fitting (DF) aspect of Cholesky decomposition, the second uses expressions obtained by differentiating the CD expression for the unperturbed two-electron integrals, while the third addresses the issue that the first two schemes are not able to represent the doubly differentiated integrals with arbitrary accuracy. This scheme uses a separate Cholesky decomposition for the cross terms in the doubly differentiated two-electron integrals.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!