AI Article Synopsis
- Mutant p53 is associated with two gain-of-function traits: chemoresistance and increased cell growth rate.
- Two methods for assessing cell sensitivity to chemotherapy and growth rate are discussed: the clonogenic assay for various chemotoxic drugs and a new long-term growth assay alternative to the MTT assay.
- Both protocols are practical, versatile, low-effort, and cost-effective, suitable for diverse cell types.
Article Abstract
Chemoresistance and increased growth rate are two gain-of-function functions that mutant p53 is thought to possess. Here, we describe two methods for measuring the sensitiveness of cells to chemotherapeutic drugs and the rate of cell growth. Both of which can be used with a wide range of cell types. The clonogenic assay can be used with many different chemotoxic drugs and the growth assay described here presents an alternative to the MTT assay and allows for a long-term measurement of cell growth. These protocols are both easy, flexible, require relatively little effort, and are inexpensive to carry out.
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| http://dx.doi.org/10.1007/978-1-62703-236-0_10 | DOI Listing |
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