Background: Obesity is a common pathology with increasing incidence, and is associated with increased mortality and healthcare costs. Several treatment options for obesity are currently available ranging from behavioral modifications to pharmaceutical agents. Many popular dietary supplements claim to enhance weight loss by acting as metabolic stimulators, however direct tests of their effect on metabolism have not been performed.
Purpose: This work identified the effects popular dietary supplements on metabolic rate and mitochondrial biosynthesis in human skeletal muscle cells.
Methods: Human rhabdomyosarcoma cells were treated with popular dietary supplements at varied doses for 24 hours. Peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α), an important stimulator of mitochondrial biosynthesis, was quantified using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Mitochondrial content was measured using flow cytometry confirmed with confocal microscopy. Glycolytic metabolism was quantified by measuring extracellular acidification rate (ECAR) and oxidative metabolism was quantified by measuring oxygen consumption rate (OCR). Total relative metabolism was quantified using WST-1 end point assay.
Results: Treatment of human rhabdomyosarcoma cells with dietary supplements OxyElite Pro (OEP) or Cellucore HD (CHD) induced PGC-1α leading to significantly increased mitochondrial content. Glycolytic and oxidative capacities were also significantly increased following treatment with OEP or CHD.
Conclusion: This is the first work to identify metabolic adaptations in muscle cells following treatment with popular dietary supplements including enhanced mitochondrial biosynthesis, and glycolytic, oxidative and total metabolism.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545995 | PMC |
| http://dx.doi.org/10.1186/1743-7075-9-101 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of Chitosan-Polyacrylic Acid Complex Systems for Enhanced Oral Delivery of and Probiotics.
Drug Des Devel Ther
January 2025
Department of Pharmaceutical Sciences and Pharmaceutics, Faculty of Pharmacy, Applied Science Private University, Amman, 11937, Jordan.
Introduction: The beneficial effects of probiotics are encountered by their low viability in gastrointestinal conditions and their insufficient stability during manufacturing, throughut the gastrointestinal transit, and storage. Therefore, novel systems are highly required to improve probiotics delivery.
Methods: In this study, Lactobacillus gasseri (L), Bifidobacterium bifidum (B), and a combination of L+B were encapsulated in chitosan (CS)-polyacrylic acid (PAA) complex systems (CS-PAA).
Editorial: Emerging technologies for viability enumeration of live microorganisms.
Front Microbiol
January 2025
Eurofins Microbiology, Inc., Madison, WI, United States.
MiCML: a causal machine learning cloud platform for the analysis of treatment effects using microbiome profiles.
BioData Min
January 2025
Department of Applied Mathematics and Statistics, The State University of New York, Korea, Incheon, South Korea.
Background: The treatment effects are heterogenous across patients due to the differences in their microbiomes, which in turn implies that we can enhance the treatment effect by manipulating the patient's microbiome profile. Then, the coadministration of microbiome-based dietary supplements/therapeutics along with the primary treatment has been the subject of intensive investigation. However, for this, we first need to comprehend which microbes help (or prevent) the treatment to cure the patient's disease.
View Article and Find Full Text PDFProbiotic active gel promotes diabetic wound healing through continuous local glucose consumption and antioxidant.
J Nanobiotechnology
January 2025
Shandong Key Laboratory of Proteins and Peptides Pharmaceutical Engineering, Shandong Universities Key Laboratory of Biological Medicine, School of Life Science and Technology, Shandong Second Medical University, 7166 # Baotong West Street, Weifang, Shandong, 261053, People's Republic of China.
Background: Diabetic foot ulcers (DFU) are severe complications of diabetes, posing significant health and societal challenges. Accumulation of reactive oxygen species (ROS) and elevated glucose levels are primary factors affecting diabetic wound healing. Achieving effective treatment by reducing ROS alone is challenging, as high glucose levels continuously drive ROS production.
View Article and Find Full Text PDFIntestine versus liver? Uncovering the hidden major metabolic organs of silybin in rats.
Drug Metab Dispos
January 2025
Jiangsu Provincial Key Laboratory of Drug Metabolism and Pharmacokinetics, Research Unit of PK-PD Based Bioactive Components and Pharmacodynamic Target Discovery of Natural Medicine of Chinese Academy of Medical Sciences, China Pharmaceutical University, Nanjing, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China. Electronic address:
Silybin, a milk thistle extract, is a flavonolignan compound with hepatoprotective effect. It is commonly used in dietary supplements, functional foods, and nutraceuticals. However, the metabolism of silybin has not been systematically characterized in organisms to date.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!