The need of cellular and sub-cellular spatial resolution in laser desorption ionization (LDI)/matrix-assisted LDI (MALDI) imaging mass spectrometry (IMS) necessitates micron and sub-micron laser spot sizes at biologically relevant sensitivities, introducing significant challenges for MS technology. To this end, we have developed a transmission geometry vacuum ion source that allows the laser beam to irradiate the back side of the sample. This arrangement obviates the mechanical/ion optic complications in the source by completely separating the optical lens and ion optic structures. We have experimentally demonstrated the viability of transmission geometry MALDI MS for imaging biological tissues and cells with sub-cellular spatial resolution. Furthermore, we demonstrate that in conjunction with new sample preparation protocols, the sensitivity of this instrument is sufficient to obtain molecular images at sub-micron spatial resolution.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jms.3108 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Repeatability of radiomic features in myocardial T1 and T2 mapping.
Eur Radiol
January 2025
Institute of Diagnostic and Interventional Radiology, Pediatric Radiology and Neuroradiology, University Medical Centre Rostock, Rostock, Germany.
Purpose: To investigate the test-retest repeatability of radiomic features in myocardial native T1 and T2 mapping.
Methods: In this prospective study, 50 healthy volunteers (29 women and 21 men, mean age 39.4 ± 13.
Proton and Carbon Ion Beam Spot Size Measurement Using 5 Different Detector Types.
Int J Part Ther
March 2025
Institute of Medical Physics and Radiation Protection, University of Applied Sciences, Giessen, Germany.
Purpose: The spot size of scanned particle beams is of crucial importance for the correct dose delivery and, therefore, plays a significant role in the quality assurance (QA) of pencil beam scanning ion beam therapy.
Materials And Methods: This study compares 5 detector types-radiochromic film, ionization chamber (IC) array, flat panel detector, multiwire chamber, and IC-for measuring the spot size of proton and carbon ion beams.
Results: Variations of up to 30% were found between detectors, underscoring the impact of detector choice on QA outcomes.
Altered interactions between attentional networks in older adults with mild cognitive impairment.
Heliyon
January 2025
School of Psychology, Shenzhen University, Shenzhen, China.
Previous research using the Attention Network Test (ANT) paradigm has indicated that older adults with mild cognitive impairment (MCI) experience declines in attentional performance across the three core networks: alerting, orienting, and executive control, primarily focusing on main effects. The present study sought to expand these findings by exploring whether interactions between these networks are also affected in the presence of MCI. To achieve this, we used the Revised Attention Network Test (ANT-R) to examine both the individual attentional networks and their interactions in 21 older adults with MCI and 27 healthy controls (HCs) matched on demographic variables.
View Article and Find Full Text PDFStudying Chinese immigrants' spatial distribution in the Raleigh-Durham area by linking survey and commercial data using romanized names.
J R Stat Soc Ser A Stat Soc
January 2025
Department of Sociology and Carolina Population Center, University of Carolina at Chapel Hill, 268 Hamilton Hall, Chapel Hill, NC 27516, USA.
Many population surveys do not provide information on respondents' residential addresses, instead offering coarse geographies like zip code or higher aggregations. However, fine resolution geography can be beneficial for characterizing neighbourhoods, especially for relatively rare populations such as immigrants. One way to obtain such information is to link survey records to records in auxiliary databases that include residential addresses by matching on variables common to both files.
View Article and Find Full Text PDFSpatially Resolved Multiomics: Data Analysis from Monoomics to Multiomics.
BME Front
January 2024
CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
Spatial monoomics has been recognized as a powerful tool for exploring life sciences. Recently, spatial multiomics has advanced considerably, which could contribute to clarifying many biological issues. Spatial monoomics techniques in epigenomics, genomics, transcriptomics, proteomics, and metabolomics can enhance our understanding of biological functions and cellular identities by simultaneously measuring tissue structures and biomolecule levels.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!