Phytotoxicity of multi-walled carbon nanotubes on red spinach (Amaranthus tricolor L) and the role of ascorbic acid as an antioxidant.

J Hazard Mater

Laboratory of Environmental Medical Chemistry, Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.

Published: December 2012

Carbon nanotubes (CNTs) are a novel nanomaterial with wide potential applications; however the adverse effects of CNTs following environmental exposure have recently received significant attention. Herein, we explore the systemic toxicity and potential influence of 0-1000 mg L(-1) the multi-walled CNTs on red spinach. The multi-walled CNTs exposed plants exhibited growth inhibition and cell death after 15 days of hydroponic culture. The multi-walled CNTs had adverse effects on root and leaf morphology, as observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Raman spectroscopy detected the multi-walled CNTs in leaves. Biomarkers of nanoparticle toxicity, reactive oxygen species (ROS), and cell damage in the red spinach were greatly increased 15 days post-exposure to the multi-walled CNTs. These effects were reversed when the multi-walled CNTs were supplemented with ascorbic acid (AsA), suggesting a role of ROS in the multi-walled CNT-induced toxicity and that the primary mechanism of the multi-walled CNTs' toxicity is oxidative stress.

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http://dx.doi.org/10.1016/j.jhazmat.2012.10.025DOI Listing

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