AI Article Synopsis
- A new multiplexed PCR assay was developed that targets the cgcA gene to identify six specific Cronobacter species.
- This new assay was tested on 305 Cronobacter isolates and showed high specificity compared to the traditional rpoB PCR method.
- The assay is expected to enhance the identification of suspected Cronobacter isolates in food-borne investigations.
Article Abstract
In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553758 | PMC |
| http://dx.doi.org/10.1128/AEM.02898-12 | DOI Listing |
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