More than a decade of research work in optofluidics has yielded a large catalogue of optofluidic elements that can manipulate light at the micro-scale (e.g., lenses, prisms). Although these elements have proven useful for many on-chip processes (e.g., miniaturized flow cytometry, interferometry and sample spectroscopy), certain deficiencies have precluded their use in micro-scale imaging. However, recent work in optofluidic imaging has avoided optofluidic elements entirely and focused instead on image capture and composition techniques, demonstrating impressive resolution in both 2D imagery and 3D tomography. In this Focus article, we will discuss some of the recent successes in optofluidic imaging and will expound our expectations for the near future of the optofluidic imaging discipline.
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http://dx.doi.org/10.1039/c2lc90127g | DOI Listing |
Nanophotonics
February 2024
Brave Analytics GmbH, Graz, Austria.
In many experiments, nanoparticles are located inside a microfluidic channel, and the light scattered by the particles becomes diffracted through the walls of the capillary. We here derive a simple but accurate approach for simulating the imaging of light through a cylindrical capillary under the assumption that the dimensions of the capillary are much larger than the wavelength of light. A comparison of the simulated images with experimental results shows very good agreement.
View Article and Find Full Text PDFNanophotonics
July 2024
The Department of Fiber Photonics, Leibniz Institute of Photonic Technology, Albert-Einstein-Street 9, 07745 Jena, Germany.
Here, we present the concept of flat-field capillary-assisted nanoparticle tracking analysis for the characterization of fast diffusing nano-objects. By combining diffusion confinement and spatially invariant illumination, i.e.
View Article and Find Full Text PDFHum Reprod
January 2025
Education Program in Reproduction and Development, EPRD, Department of obstetrics and Gynaecology, Monash University, Clayton, VIC, Australia.
Study Question: Is it feasible to safely determine metabolic imaging signatures of nicotinamide adenine dinucleotide [NAD(P)H] associated auto-fluorescence in early embryos using a light-sheet on-a-chip approach?
Summary Answer: We developed an optofluidic device capable of obtaining high-resolution 3D images of the NAD(P)H autofluorescence of live mouse embryos using a light-sheet on-a-chip device as a proof-of-concept.
What Is Known Already: Selecting the most suitable embryos for implantation and subsequent healthy live birth is crucial to the success rate of assisted reproduction and offspring health. Besides morphological evaluation using optical microscopy, a promising alternative is the non-invasive imaging of live embryos to establish metabolic activity performance.
J Cell Sci
October 2024
Biomedical Imaging Group and Center for Imaging, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Adv Sci (Weinh)
September 2024
Bionanophotonic Systems Laboratory, Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, 1015, Switzerland.
Organoid tumor models have emerged as a powerful tool in the fields of biology and medicine as such 3D structures grown from tumor cells recapitulate better tumor characteristics, making these tumoroids unique for personalized cancer research. Assessment of their functional behavior, particularly protein secretion, is of significant importance to provide comprehensive insights. Here, a label-free spectroscopic imaging platform is presented with advanced integrated optofluidic nanoplasmonic biosensor that enables real-time secretion analysis from single tumoroids.
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