[Effect of insulin on prohibitin in the osteogenic differentiation of rat's mandibular bone marrow stromal cells in high glucose].

Purpose: To explore the effect of insulin on the expression of prohibitin in osteogenic differentiation of rat's mandibular bone marrow stromal cells(BMSC) in high glucose.

Methods: Bone marrow stromal cells were obtained from the mandible of Wistar rats and stimulated in three glucose concentrations mineral medium(5.5, 25, 45 mM) with or without insulin(10-5 M) for 1, 3, 7,14 and 21 days. The expression of prohibitin was quantified via enzyme-linked immunosorbent assays (ELISA). The mineralization nodules assessment was performed at day 21 by alizarine red staining. The statistics analysis was performed using SPSS 15.0 software package.

Results: High glucose of 45 mM inhibited mineralization of rBMSC and insulin could improve the mineralization in high glucose. The expression of prohibitin of 45 mM group decreased significantly compared with 5.5 mM group and 25 mM group. The expression of prohibitin of each group achieved maximum at day 3. Insulin (10-5 M) increased the expression of prohibitin of 25 mM group and 45 mM group in osteogenic differentiation of rBMSC.

Conclusions: High glucose inhibited the expression of prohibitin in osteogenic differentiation of rBMSC, and insulin can improve the effect. Prohibitin may play an important part in early stage in osteogenic differentiation of rBMSC.