Purpose: With the observation of bacterial adhesion on the surface of the PFM and enamel, we analyzed the sequence of these adhesions and offer options for prevention of their occurrence.
Methods: The specimen made of PFM were polished step by step. Then they, along with the enamel were put into the liquid BHI culture medium containing Ua, Pg and An. After 6h, 12h and 24h the adhesive microbial amount in the culture medium was determined by counting formation unit. F test was used to analyze the results with SPSS 6.0 software package.
Results: Pg was found neither on the surface of specimen, nor in the liquid BHI culture medium; The value of Ua/An in the groups of PFM was less than that in the groups of enamel within 6 hours. And 12 hours later, the maximal increment of An was seen in the group of PFM. And there was no change in the groups of PFM and enamel during 12 and 24 hours.
Conclusions: Pg was inhibited when these bacteria were cultured together. If we wish to prevent the adhesion of Ua and An, the first 12 hours is the critical period for PFM. Given the result of the analysis, PFM is a good restoration.
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J Biomed Mater Res A
January 2025
Faculty of Materials Science and Engineering, Warsaw University of Technology, Warsaw, Poland.
Bone tissue regeneration can be affected by various architectonical features of 3D porous scaffold, for example, pore size and shape, strut size, curvature, or porosity. However, the design of additively manufactured structures studied so far was based on uniform geometrical figures and unit cell structures, which often do not resemble the natural architecture of cancellous bone. Therefore, the aim of this study was to investigate the effect of architectonical features of additively manufactured (aka 3D printed) titanium scaffolds designed based on microtomographic scans of fragments of human femurs of individuals of different ages on in vitro response of human bone-derived mesenchymal stem cells (hMSC).
View Article and Find Full Text PDFFront Plant Sci
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Department of Environmental Sciences, Southern Illinois University Edwardsville, Edwardsville, IL, United States.
This study aimed at quantifying the potential effects of plant and soil microbial interaction on selenium (Se) volatilization, with the specific objectives of identifying soil bacteria associated with rabbitfoot grass () and demonstrating the enhancement of Se volatilization in the soil-Indian mustard () system through inoculation of the soil with the identified best Se-volatilizing bacterial strain. Soil bacteria were isolated from topsoil and rhizosphere soils of rabbitfoot grass, and the bacterial colonies were characterized via PCR-DGGE and DGGE band analysis prior to their identification using 16S rDNA sequencing technique. produced over 500-fold more volatile Se in a culture medium treated with 15 µg Se/mL (equal mixture of SeO , SeO and selenomethionine) than any of the other eight identified bacterial strains.
View Article and Find Full Text PDFBackground: Cachexia is defined by chronic loss of fat and muscle, is a frequent complication of pancreatic ductal adenocarcinoma (PDAC), and negatively impacts patient outcomes. Nutritional supplementation cannot fully reverse tissue wasting, and the mechanisms underlying this phenotype are unclear. This work aims to define the relative contributions of catabolism and anabolism to adipose wasting in PDAC-bearing mice.
View Article and Find Full Text PDFJ Biomed Mater Res B Appl Biomater
January 2025
Department of Mechanical Engineering, Cleveland State University, Cleveland, Ohio, USA.
Osteoarthritis (OA) is a prevalent joint disorder that is characterized by the degeneration of articular cartilage in synovial joints. Most of the current treatment options for this disorder tend to focus on symptom management rather than addressing the underlying progression of the disease. Cartilage tissue engineering has emerged as a promising approach to address the limitations of current OA treatments, aiming to regenerate cartilage and restore the natural function of affected joints.
View Article and Find Full Text PDFJ Exp Clin Cancer Res
January 2025
National-Local Joint Engineering Laboratory of Druggability and New Drug Evaluation, Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, China.
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