Background: A number of methods for detecting diversity in Entamoeba have been described over the years. In the present study the genetic polymorphism of noncoding locus A-L was analyzed using PCR and sequencing in order to clarify the genotypic differences among E. dispar isolates.
Methods: A total of 28 E. dispar from patients with gastrointestinal symptoms were determined and the genomic DNA was extracted directly from stool. For genotype analysis; Locus A-L was amplified by PCR and PCR products were sequenced. The sequences obtained were edited manually and aligned using Gene Runner software.
Results: With sequencing of PCR products a reliable genetic diversity in size, number and position of the repeat units were observed among the Iranian E. dispar isolates in locus A-L gene. Sequences showed variation in length from 448bp to 507bp and seven distinct types were identified.
Conclusion: The genetic diversity of loci like A-L shows them to be suitable for epidemiological studies such as the characterization of the routes of transmission of these parasites in Iran.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3488828 | PMC |
Publication Analysis
Top Keywords
Similar Publications
Unearthing Genetic Treasures: Exploring Lost Autochthonous Varieties in Lebanon.
Genes (Basel)
December 2024
Laboratoire Biodiversité et Génomique Fonctionnelle, Faculté des Sciences, Université Saint-Joseph, Campus Sciences et Technologies, Mar Roukos, Mkalles, P.O. Box 1514, Riad el Solh, Beirut 1107 2050, Lebanon.
Background/objectives: Lebanon, one of the oldest centers of grapevine ( L.) cultivation, is home to a rich diversity of local grape varieties. This biodiversity is linked to the country's unique topography and millennia of cultural history.
View Article and Find Full Text PDFIL1RAP Blockade With a Monoclonal Antibody Reduces Cardiac Inflammation and Preserves Heart Function in Viral and Autoimmune Myocarditis.
Circ Heart Fail
December 2024
Department of Pathology, School of Medicine (D.A.L., D.E., M.V.T., D.Č.), Johns Hopkins University, Baltimore, MD.
Background: Currently, there are no therapies targeting specific pathogenic pathways in myocarditis. IL (interleukin)-1 blockade has shown promise in preclinical studies and case reports. We hypothesized that blockade of IL1RAP (IL-1 receptor accessory protein), a shared subunit of the IL-1, IL-33, and IL-36 receptors, could be more efficient than IL-1 blockade alone.
View Article and Find Full Text PDFNOTCH3 p.Arg1231Cys is markedly enriched in South Asians and associated with stroke.
Nat Commun
September 2024
Columbia University, New York, NY, USA.
Article Synopsis
- The genetic factors contributing to stroke risk in South Asians remain largely unstudied, with a recent study examining 75,000 Pakistanis using exome-wide sequencing.
- A specific genetic variant, NOTCH3 p.Arg1231Cys, was found to be more common in South Asians (0.58%) compared to Western Europeans (0.019%) and was significantly linked to hemorrhagic and overall stroke risk.
- This variant accounts for about 2.0% of hemorrhagic strokes and 1.1% of all strokes in South Asians, emphasizing the importance of including diverse populations in genetic research for better understanding and treatment of stroke.
Preclinical validation of an O-antigen glycoconjugate for the prevention of serotype O1 invasive disease.
Microbiol Spectr
June 2024
Pfizer Vaccine Research and Development, Pearl River, New York, USA.
Unlabelled: A US collection of invasive serotype O1 bloodstream infection (BSI) isolates were assessed for genotypic and phenotypic diversity as the basis for designing a broadly protective O-antigen vaccine. Eighty percent of the BSI isolate serotype O1 strains were genotypically ST95 O1:K1:H7. The carbohydrate repeat unit structure of the O1a subtype was conserved in the three strains tested representing core genome multi-locus sequence types (MLST) sequence types ST95, ST38, and ST59.
View Article and Find Full Text PDFCryptic diversity and virulence of recovered from (spotted lanternfly) in eastern Pennsylvania.
Front Insect Sci
April 2023
Department of Entomology, Cornell University, Ithaca, NY, United States.
The entomopathogenic fungus is cosmopolitan and known to infect a variety of sap-sucking pests like aphids, mealybugs, and scales in the order of Hemiptera. In Fall 2017, spotted lanternfly (SLF) adults killed by the fungal entomopathogen were found in Berks County, Pennsylvania. In 2018-2020 we collected SLF and nearby non-target insects killed by spp.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!