In this study, the effects of heavy metals (Cd, Cu, Pb, Zn) on EROD and CYP3A4 activities in the earthworm Eisenia fetida were evaluated to find out their possible induction and potential as biomarkers for soil heavy metal contamination. The earthworms were exposed to increasing concentrations of Cd (0.1-8 mg L(-1)), Cu (10-200 mg L(-1)), Pb (20-400 mg L(-1)) or Zn (50-400 mg L(-1)) in filter papers for 48 h. EROD activity was significantly changed in dose-dependent manners after exposure to each of the four metals. CYP3A4 activity was significantly induced by Cd and Pb, rather by Cu and Zn. This is the first report on heavy metal-induced changes of CYP3A4 activity in earthworms. Among the four heavy metals, Cd was the most potent inducing EROD and CYP3A4. While EROD and CYP3A4 activities showed a similar trend, EROD is more sensitive than CYP3A4 activity in E. fetida as a biomarker for heavy metals pollution.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jhazmat.2012.10.012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Human Cytochrome P450 Cancer-Related Metabolic Activities and Gene Polymorphisms: A Review.
Cells
November 2024
Independent Researcher, 108815 Moscow, Russia.
Background: Cytochromes P450 (CYPs) are heme-containing oxidoreductase enzymes with mono-oxygenase activity. Human CYPs catalyze the oxidation of a great variety of chemicals, including xenobiotics, steroid hormones, vitamins, bile acids, procarcinogens, and drugs.
Findings: In our review article, we discuss recent data evidencing that the same CYP isoform can be involved in both bioactivation and detoxification reactions and convert the same substrate to different products.
Machine Learning-Based Prediction of the Inhibitory Activity of Chemical Substances Against Rat and Human Cytochrome P450s.
Chem Res Toxicol
November 2024
Laboratory of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 4228526, Japan.
The prediction of cytochrome P450 inhibition by a computational (quantitative) structure-activity relationship approach using chemical structure information and machine learning would be useful for toxicity research as a simple and rapid tool. However, there are few models focusing on the species differences between rat and human in the P450s inhibition. This study aimed to establish models to classify chemical substances as inhibitors or non-inhibitors of various rat and human P450s, using only molecular descriptors.
View Article and Find Full Text PDFCombinatory Effects of Acrylamide and Deoxynivalenol on In Vitro Cell Viability and Cytochrome P450 Enzymes of Human HepaRG Cells.
Toxins (Basel)
September 2024
Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, 1090 Vienna, Austria.
Article Synopsis
- Acrylamide (AA) forms when carbohydrate-rich foods are heated, while deoxynivalenol (DON) is a mycotoxin that contaminates many cereal products, leading to potential combined exposure in diets.
- A study investigated the effects of different concentrations of AA and DON on cell toxicity and the expression of certain liver enzymes in human liver cells, finding that DON primarily drove cytotoxic effects.
- Results showed that while low levels of DON increased certain gene expressions, no significant harmful interactions between AA and DON were observed, suggesting that their combination does not have a major impact on liver cell function.
Cadmium causes spleen toxicity in chickens by regulating mitochondrial unfolded protein response and nuclear receptors response.
Poult Sci
November 2024
Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, China; Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Department of Clinical Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Cadmium (Cd) is a heavy metal that pollutes the environment and threatens human and animal health via the food chain. The spleen is one of the target organs affected by Cd toxicity. However, the mechanism of Cd toxicity is not fully understood.
View Article and Find Full Text PDFGal-1-mediated cytochrome p450 activation promotes fibroblast into myofibroblast differentiation in pulmonary fibrosis.
Int Immunopharmacol
November 2024
Department of General Practice, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China; Wenzhou Key Laboratory of Precision General Practice and Health Management, Wenzhou 325000, China; South Zhejiang Institute of Radiation Medicine and Nuclear Technology, Wenzhou 325014, China; Department of General Practice, Taizhou Women and Children's Hospital of Wenzhou Medical University, Taizhou 318001, China. Electronic address:
Pulmonary fibrosis (PF) results from excessive extracellular matrix (ECM) deposition and tissue remodeling after activation of fibroblasts into myofibroblasts. Abnormally deposited fibrotic ECM, in turn, promotes fibroblast activation and accelerates loss of lung structure and function. However, the molecular mediators and exact mechanisms by which fibrotic ECM promotes fibroblast activation are unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!