Monocyte chemoattractant protein 1 (MCP-1) is a CC chemokine which stimulates mononuclear leukocytes and is significant in the pathogenesis of inflammatory disease. Curcumin, extracted from Curcumae longae, has been shown to possess anti-inflammatory activity, in inflammation associated with the induction of MCP-1 expression. However, the underlying mechanisms involved in the effect of curcumin on MCP-1 expression remain unclear. In the current study, we investigated the effect of curcumin on the production of MCP-1 induced by lipopolysaccharide (LPS) in macrophages and the possible mechanisms involved. The results revealed that curcumin decreased MCP-1 production in a concentration-dependent manner and reduced the generation of reactive oxygen species (ROS) induced by LPS in RAW264.7 macrophages. Additionally, zinc protoporphyrin, a heme oxygenase-1 (HO-1) inhibitor, blocked the inhibitory effect of curcumin on the LPS-induced MCP-1 expression. The exposure of cells to curcumin was found to enhance HO-1 expression. Furthermore, additional experiments indicated that the inhibitory effect of curcumin on LPS-induced MCP-1 expression was significantly attenuated in the presence of N-acetylcysteine (an effective ROS scavenger). The results presented in our study suggest that curcumin enhances the expression of HO-1 to reduce the LPS-induced production of ROS, which leads to the inhibition of MCP-1 expression in RAW264.7 macrophages.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3892/mmr.2012.1138 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Hypercholesterolemia triggers innate immune imbalance and transforms brain infarcts after ischemic stroke.
Front Immunol
January 2025
Institute for Experimental Immunology and Imaging, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Post-stroke early activation of neutrophils contributes to intensive neuroinflammation and worsens disease outcomes. Other pre-existing patient conditions can modify the extent of their activation during disease, especially hypercholesterolemia. However, whether and how increased circulating cholesterol amounts can change neutrophil activation responses very early after stroke has not been studied.
View Article and Find Full Text PDFDesign and development of an isatin-1,2,3-triazole hybrid analogue as a potent anti-inflammatory agent with enhanced efficacy and gene expression modulation.
RSC Adv
January 2025
Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad India.
Isatin (1-indole-2,3-dione) and its derivatives have been found to exhibit various biological activities, including anticancer and antidiabetic properties. In this study, a series of nine isatin-1,2,3-triazole conjugates were synthesized and evaluated for their anti-inflammatory potential experiments. Their synthesis involved the propargylation of isatin 1 with propargyl bromide to obtain -propargyl isatin 2, which was subjected to click reactions with different aromatic azides to yield isatin--1,2,3-triazoles (3a-i).
View Article and Find Full Text PDFNew formulation of ibuprofen-arginate reduces oxidative stress and prevents macrophage polarization toward M1 phenotype.
Biomed Pharmacother
January 2025
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), Argentina; Universidad Nacional de Cuyo, Facultad de Ciencias Médicas, Instituto de Bioquímica y Biotecnología, Avda. Libertador 80, Mendoza CP5500, Argentina. Electronic address:
A hypertonic solution of Ibuprofen (Ibu) was designed to nebulize, associating a low concentration of Ibu with L-Arginine (AR), to increase solubility and serve as a nitric oxide donor. To provide preclinical research human bronchial epithelial cells derived from a cystic fibrosis patient homozygous for the ΔF508 CFTR mutation (CFBE41o-) and mouse RAW 264.7 macrophages were pre-treated with Ibu (10-100 μM), AR (20 and 200 μM), or the combination Ibu-AR (10-100 μM).
View Article and Find Full Text PDFChemokine associations with blood cerebrospinal fluid (CSF) barrier permeability and delirium.
Brain Behav Immun Health
February 2025
School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute & Trinity College Institute of Neuroscience, Trinity College Dublin, Pearse Street, Dublin 2, Ireland.
Delirium is a highly prevalent neuropsychiatric syndrome characterised by acute and fluctuating impairments in attention and cognition. Mechanisms driving delirium are poorly understood but it has been suggested that blood cytokines and chemokines cross the blood brain barrier during delirium, directly impairing brain function. It is not known whether these molecules reach higher brain levels when the blood cerebrospinal fluid barrier (BCSFB) is impaired.
View Article and Find Full Text PDFAsperulosidic acid inhibits the PI3K/Akt/NF-κB pathway to suppress endotoxin-induced uveitis.
Front Med (Lausanne)
January 2025
Department of Ophthalmology, Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Eye Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Introduction: Uveitis, a severe inflammatory disease affecting the uvea, is associated with visual impairment and irreversible blindness. Asperulosidic Acid (ASPA), derived from , is known for its notable anti-inflammatory and antioxidant characteristics.
Methods: The present study explored the potential anti-inflammatory effects and the fundamental processes of ASPA by injecting it or a placebo into the vitreous of rats with endotoxin-induced uveitis (EIU).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!