Targeted transgenesis using site-specific recombinases is an attractive method to create genetically modified animals as it allows for integration of the transgene in a pre-selected transcriptionally active genomic site. Here we describe the application of recombinase-mediated cassette exchange (RMCE) in cells from a Göttingen minipig with four RMCE acceptor loci, each containing a green fluorescence protein (GFP) marker gene driven by a human UbiC promoter. The four RMCE acceptor loci segregated independent of each other, and expression profiles could be determined in various tissues. Using minicircles in RMCE in fibroblasts with all four acceptor loci and followed by SCNT, we produced piglets with a single copy of a transgene incorporated into one of the transcriptionally active acceptor loci. The transgene, consisting of a cDNA of the Alzheimer's disease-causing gene PSEN1M146I driven by an enhanced human UbiC promoter, had an expression profile in various tissues similar to that of the GFP marker gene. The results show that RMCE can be done in a pre-selected transcriptionally active acceptor locus for targeted transgenesis in pigs.
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http://dx.doi.org/10.1007/s11248-012-9671-6 | DOI Listing |
Theor Appl Genet
November 2024
State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Jiangsu Nanjing National Field Scientific Observation and Research Station for Rice Germplasm, Key Laboratory of Biology, Genetics and Breeding of Japonica Rice in Mid-lower Yangtze River, Ministry of Agriculture and Rural Affairs, Sanya Research Institute, Nanjing Agricultural University, Nanjing, 210095, China.
Key message A major quantitative trait locus (qGW12) for grain shape and weight has been isolated in rice, corresponding to LOC_Os12g17900/OsPUB23, and its encoded protein interacts with OsMADS1. Grain shape in rice is an important trait that influences both yield and quality. The primary determinants of grain shape are quantitative trait loci (QTLs) inherited from natural variation in crops.
View Article and Find Full Text PDFAnimals (Basel)
October 2024
Department of Agricultural, Forest and Food Sciences, University of Turin, 10095 Grugliasco, TO, Italy.
The αs2-casein is a phosphoprotein secreted in the milk of most mammals, and it is the most hydrophilic of all caseins. Contrary to genes found in ruminants, in donkeys two different encoding genes for donkey αs2-casein ( I and II) have been identified. However, unlike in ruminants, the variability at these loci has not been characterized in detail in donkeys until now.
View Article and Find Full Text PDFNucleic Acids Res
November 2024
UMR CNRS7276, Inserm1262, Université de Limoges: Contrôle de la Réponse Immune B et des Lymphoproliférations, Team 2, B-NATION: B Cell Nuclear Architecture, Immunoglobulin Genes and Oncogenes, 2, rue du Dr. Marcland, 87025 Limoges, France.
In B lymphocytes, class switch recombination (CSR) is an essential process that adapts immunoglobulin (Ig) subtypes to antigen response. Taking place within the Ig heavy chain (IgH) locus, CSR needs controlled transcription of targeted regions governed by the IgH 3' regulatory region (3'RR). This super-enhancer is composed of four core enhancers surrounded by inverted repeated sequences, forming a quasi-palindrome.
View Article and Find Full Text PDFFront Cardiovasc Med
July 2024
Department of Cardiology, Xianyang Hospital, Yan'an University, Xianyang, China.
Background: Atrial fibrillation (AF) is a common persistent arrhythmia characterized by rapid and chaotic atrial electrical activity, potentially leading to severe complications such as thromboembolism, heart failure, and stroke, significantly affecting patient quality of life and safety. As the global population ages, the prevalence of AF is on the rise, placing considerable strains on individuals and healthcare systems. This study utilizes bioinformatics and Mendelian Randomization (MR) to analyze transcriptome data and genome-wide association study (GWAS) summary statistics, aiming to identify biomarkers causally associated with AF and explore their potential pathogenic pathways.
View Article and Find Full Text PDFNew Phytol
November 2024
Department of Biochemistry, University of Cambridge, Hopkins Building, The Downing Site, Tennis Court Road, Cambridge, CB2 1QW, UK.
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