AI Article Synopsis
- The study developed microsatellite markers in Trifolium montanum to examine pollen dispersal patterns using both indirect and direct paternity analyses.
- Screening of existing markers yielded four usable ones, while seven new markers were created, all showing high variability in populations sampled.
- The findings support the effectiveness of these markers as reliable tools for population genetics and parentage research in this grassland species.
Article Abstract
Premise Of The Study: Microsatellite markers were developed in Trifolium montanum to investigate pollen dispersal patterns at landscape scale with a pollen-pool analysis (indirect parentage analysis) as well as in an experimental set-up with a direct paternity analysis.
Methods And Results: Screening 46 microsatellites developed for T. repens yielded four markers usable in T. montanum. Seven additional ones have been developed specifically for the target species using a 454-sequencing approach. All markers were polymorphic, with an allele number ranging from two to 45 based upon 254 individuals sampled from four populations, and an exclusion probability of 0.999.
Conclusions: These markers proved a useful and reliable molecular tool for use in population genetics and parentage studies of this common grassland herb.
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| http://dx.doi.org/10.3732/ajb.1200102 | DOI Listing |
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