The complete genome sequence of Bacillus subtilis strain QB928 was constructed to facilitate studies in the evolution of the genetic code. With a widespread use of the strain in Bacillus subtilis genetics studies, its complete genome sequence would facilitate deeper understanding of Bacillus subtilis genetics.
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http://dx.doi.org/10.1128/JB.01533-12 | DOI Listing |
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June 2025
Department of Civil Engineering, SIT, Symbiosis International Deemed University, Pune, India.
Numerous problems, including weathering, cracks, leaks, and bending, among others, appear in concrete after construction. The optimum procedure for treating concrete cracks is the subject of this study. The research goal is to find potential crack treatment materials and analyze the effect on the properties of concrete caused by the addition of bacterial solution to the concrete mix along with a particular method of preparation of bacterial solution.
View Article and Find Full Text PDFMicrob Cell Fact
January 2025
Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.
Background: Ribosome pausing slows down translation and can affect protein synthesis. Improving translation efficiency can therefore be of commercial value. In this study, we investigated whether ribosome pausing occurs during production of the α-amylase AmyM by the industrial production organism Bacillus subtilis under repeated batch fermentation conditions.
View Article and Find Full Text PDFFish Shellfish Immunol
January 2025
Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, People's Republic of China. Electronic address:
The Chinese mitten crab (Eriocheir sinensis) is an important component in Chinese aquaculture. Due to its lacking adaptive immune system as a crustacean, it exhibits poor tolerance to environmental stresses, particularly the deleterious impact of lipopolysaccharide (LPS) from pathogenic bacteria during E. sinensis culture.
View Article and Find Full Text PDFEnzyme Microb Technol
January 2025
Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Bioconversion and Bioseparation for Platform Chemical Production, Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Bangkok 10330, Thailand; Water Science and Technology for Sustainable Environment Research Unit, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address:
Inducer-free expression systems are promising tools for biorefinery because they can reduce the reliance on inducers, reducing production costs and simplifying processes. Owing to their broad range of substrate structures and catalytic reactions, cytochrome P450s are promising biocatalysts to produce value-added compounds. However, unsuitable levels of cytochrome P450 expression could result in cell stress, affecting the efficiency of the biocatalyst.
View Article and Find Full Text PDFProtein Expr Purif
January 2025
Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an, 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, 710069, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an, 710069, China. Electronic address:
Ginseng peptides, small molecule active ingredients in ginseng, are mainly extracted naturally or synthesised chemically, but high costs and difficulties hinder further research. In this study, a ginseng hexapeptide FKEHGY, named antitumor peptide 0601 (AT0601) and its five tandem sequence repeats AT0605, were expressed in Bacillus subtilis WB600 for the first time, and the bioactivity study showed that the anticancer activity of AT0605 was even significantly higher than that of AT0601 for colon cancer CT26 cells, with IC50s of 16.82±1.
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