A new method consisting of a two-step activation was developed in order to covalently immobilize enzymes on calibrate nylon 66 spheres. This efficient method associates for the first time peptide bond cleavage and O-alkylation of the support. Optimal conditions for activation and protein coupling were defined, and immobilized trypsin was used to investigate the histone accessibility on chromatin. This approach, which allows us to degrade first progressively H1, indicates that H4 seems inaccessible both in relaxed and condensed chromatin.
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http://dx.doi.org/10.1016/0006-291x(90)91722-5 | DOI Listing |
Environ Pollut
January 2025
Department of Materials Science and Engineering, University of Michigan, 2300 Hayward Street, Ann Arbor, MI, 48109-2117, USA. Electronic address:
Experimental efforts supplemented by modeling gauged whether common additives found in soaps and laundry detergents interfered with polyacrylate adhesive-based capture of microplastics. On the experimental front, poly(2-ethylhexyl acrylate) (PEHA) samples were evaluated using gravimetric analysis, probe tack, and functional assessments of adhesive-coated glass slides immersed into DI water solutions containing both microparticles and additives (solvents, softeners, and non-ionic surfactants). Nylon-6 spheres and polyethylene terephthalate microplastics were chosen for adsorption using a count-based method by ImageJ imaging analysis.
View Article and Find Full Text PDFBMC Urol
May 2024
Department of Urology, Giresun University Faculty of Medicine, Giresun, Turkey.
Background: Microplastics are ubiquitous, widespread environmental pollutants with unavoidable human exposure. Herein, it was aimed to investigate the presence of microplastics in prostate tissue.
Methods: Prostate tissues from 12 patients who underwent Trans Urethral Resection of the Prostate (TUR-P) were analyzed to investigate the presence of microplastics.
Environ Pollut
September 2023
Instituto Politécnico Nacional (IPN), Centro Mexicano para la Producción más Limpia (CMP+L), Av. Acueducto s/n, Col. Barrio la Laguna Ticomán, Del Gustavo A. Madero, C.P. 07340, México City, Mexico.
Inadvertent human exposure to microplastics by the ingestion of microplastic-contaminated processed foods poses health risks and new preventative issues; nevertheless, investigations analyzing microplastic occurrences in commercially dried fish for direct human consumption are scarce. This study assessed the abundance and characteristics of microplastics in 25 commercially sold dried fish products (4 supermarkets, 3 street vendors, and 18 traditional agri-product farmers' markets) from two widely consumed and commercially important Chirostoma species (C. jordani and C.
View Article and Find Full Text PDFEnviron Sci Technol
November 2022
Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340, United States.
Ultrasound Med Biol
January 2023
School of Biomedical Engineering, Western University, London, Ontario, Canada; Robarts Research Institute, Western University, London, Ontario, Canada.
Joint arthropathies often require continuous monitoring of the joint condition, typically performed using magnetic resonance (MR) or ultrasound (US) imaging. US imaging is often the preferred screening or diagnostic tool as it is fast and inexpensive. However, conventional 2-D US has limited capability to compare imaging results between examinations because of its operator dependence and challenges related to repeat imaging in the same location and orientation.
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