AI Article Synopsis
- Novel biomarkers for laryngeal squamous cell carcinoma (LSCC) are urgently needed, leading to the identification of multiple associated genes using microarray expression profiling.
- The gene DCUN1D5 showed high expression in LSCCs, and its overexpression increased cell migration, invasion, and proliferation significantly in laboratory tests.
- After DNA damage, DCUN1D5 levels decreased, and reducing its expression led to less cell division and more cell death, suggesting it plays a critical role in the DNA damage response.
Article Abstract
Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes.
Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro.
Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%.
Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.
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| http://dx.doi.org/10.7314/apjcp.2012.13.8.4157 | DOI Listing |
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