AI Article Synopsis
- The World Health Organization has added flow cytometry to help diagnose myelodysplastic syndromes (MDS), focusing on abnormal antigen expression in bone marrow blasts to identify low-grade MDS.
- This study analyzed 175 patients to compare antigen expression in CD34+ bone marrow cells from those diagnosed with MDS versus those with secondary cytopenia, including markers like CD34, CD117, and CD123.
- Findings showed that a high percentage of CD34+ cells and certain overexpressed markers, along with abnormal CD45 expression, serve as effective indicators for MDS and reflect similarities with CD34+ acute myeloid leukemia (AML).
Article Abstract
The World Health Organization introduced flow cytometry as an additional criterion for diagnosis of myelodysplastic syndromes (MDS). Aberrant antigen expression on bone marrow (BM) blasts may identify "low-grade MDS." This study aimed to examine differences in antigen expression on CD34+ BM cells between patients with MDS and those with secondary cytopenia. BM aspirates of 175 patients with cytopenia were classified as MDS or secondary cytopenia. Expression of stem cell antigens (CD34, CD133), myeloid antigens (CD13, CD33), B-cell antigens (CD19, CD10), growth factor receptors (CD117, CD123), and chemokine receptor (CD184) was examined. Thirty-two normal adults and 49 patients with CD34+ acute myeloid leukemia (AML) were also examined. High percentage of CD34+ cells, CD117 and CD123 overexpression, and abnormal CD45 expression on these cells are the best markers for MDS. These phenotypic aberrancies correlate with number of blasts and degree of dysplasia, and were similar to those in CD34+ AML, thus reflecting the relationship between these disorders.
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| http://dx.doi.org/10.1309/AJCPAGVO27RPTOTV | DOI Listing |
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