AI Article Synopsis
- Worniu, a Snail family transcription factor in Drosophila, is crucial for regulating neuroblast development and preventing premature differentiation.
- Mutants lacking Worniu display defects in neuroblast delamination and have altered gene expression, showing reduced cell-cycle-related genes and increased differentiation genes.
- The study finds that Worniu is essential for maintaining neuroblast self-renewal by promoting cell-cycle progression and inhibiting early differentiation through the modulation of the splicing factor Elav.
Article Abstract
Snail family transcription factors are best known for regulating epithelial-mesenchymal transition (EMT). The Drosophila Snail family member Worniu is specifically transcribed in neural progenitors (neuroblasts) throughout their lifespan, and worniu mutants show defects in neuroblast delamination (a form of EMT). However, the role of Worniu in neuroblasts beyond their formation is unknown. We performed RNA-seq on worniu mutant larval neuroblasts and observed reduced cell-cycle transcripts and increased neural differentiation transcripts. Consistent with these genomic data, worniu mutant neuroblasts showed a striking delay in prophase/metaphase transition by live imaging and increased levels of the conserved neuronal differentiation splicing factor Elav. Reducing Elav levels significantly suppressed the worniu mutant phenotype. We conclude that Worniu is continuously required in neuroblasts to maintain self-renewal by promoting cell-cycle progression and inhibiting premature differentiation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3509944 | PMC |
| http://dx.doi.org/10.1016/j.devcel.2012.09.007 | DOI Listing |
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