Chemical reporters are unique functional groups that can be used to label biomolecules in living systems. Only a handful of broadly applicable reporters have been identified to date, owing to the rigorous demands placed on these functional groups in biological settings. We describe here a new chemical reporter-cyclopropene-that can be used to target biomolecules in vitro and in live cells. A variety of substituted cyclopropene scaffolds were synthesized and found to be stable in aqueous solution and in the presence of biological nucleophiles. Furthermore, some of the cyclopropene units were metabolically introduced into cell surface glycans and subsequently detected with covalent probes. The small size and selective reactivity of cyclopropenes will facilitate efforts to tag diverse collections of biomolecules in vivo.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ja3060436 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism.
Environ Int
January 2025
Department of Agricultural Biotechnology, Seoul National University, Seoul 00826, Republic of Korea; Department of Food and Animal Biotechnology, Seoul National University, Seoul 00826, Republic of Korea; Center for Food and Bioconvergence, Research Institute for Agricultural and Life Sciences, Interdisciplinary Programs in Agricultural Genomics, Seoul National University, Seoul 00826, Republic of Korea; Green Bio Science & Technology, Bio-Food Industrialization, Seoul National University, 1447 Pyeongchang-daero, Daehwa-myeon, Pyeongchang-gun, Gangwon-do 25354, Republic of Korea. Electronic address:
Hepatic metabolism is an important process for evaluate the potential activity and toxicity of endocrine disrupting chemicals (EDCs) metabolites. Organization for Economic Co-operation and Development (OECD) has advocated the development of in vitro assays that mimic in vivo hepatic metabolism to eventually replace classical animal tests. In response to this need, we established a 3D mouse liver organoid (mLO) platform that mimics the animal model and is distinct from existing models.
View Article and Find Full Text PDFConventional and Tropism-Modified High-Capacity Adenoviral Vectors Exhibit Similar Transduction Profiles in Human iPSC-Derived Retinal Organoids.
Int J Mol Sci
December 2024
Department of Ophthalmology, Leiden University Medical Center (LUMC), Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
Viral vector delivery of gene therapy represents a promising approach for the treatment of numerous retinal diseases. Adeno-associated viral vectors (AAV) constitute the primary gene delivery platform; however, their limited cargo capacity restricts the delivery of several clinically relevant retinal genes. In this study, we explore the feasibility of employing high-capacity adenoviral vectors (HC-AdVs) as alternative delivery vehicles, which, with a capacity of up to 36 kb, can potentially accommodate all known retinal gene coding sequences.
View Article and Find Full Text PDFQuercetin can improve anesthesia induced neuroinflammation and cognitive dysfunction by regulating miR-138-5p/ LCN2.
BMC Anesthesiol
January 2025
Department of Neurosurgery, Xijing Hospital, No. 127, Changle West Road, Xi'an, 710032, China.
Background: Anesthesia can lead to functional cognitive impairment, which can seriously affect postoperative recovery. To investigate the effect and mechanism of quercetin (Que) in anesthetized rats, the study provided a new therapeutic idea for the prevention of cognitive dysfunction caused by anesthesia.
Methods: Cognitively impaired rats were constructed using Isoflurane (ISO) anesthesia and treated with Que.
Template-assembly activation of primer exchange reaction for on-site and sensitive detection of copper ions in blood.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
The First Clinical Medical College, Shandong University of Traditional Chinese Medicine, Jinan, 250355, PR China; Department of Pharmacy, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, PR China. Electronic address:
The sensitive and accurate detection of copper ions is crucial for public health, medical research, and environmental monitoring. In this study, we developed a sensor based on template-assembly activation of the primer exchange reaction (PER) for the on-site detection of copper ions in blood. Copper ions triggered the assembly of two template fragments into a hairpin structure via a click-chemistry reaction, activating the PER.
View Article and Find Full Text PDFProbing the orientation and membrane permeation of rhodamine voltage reporters through molecular simulations and free energy calculations.
J Mater Chem B
January 2025
Department of Physics, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Article Synopsis
- The transmembrane potential is crucial for cellular functions like signaling and energy production, with Rhodamine voltage reporters (RhoVRs) serving as small, non-invasive sensors that can detect voltage changes, especially in mitochondria.
- Extensive simulations and free-energy calculations revealed that the orientation of RhoVRs relative to membranes, influenced by their polarized functional groups, significantly impacts their voltage sensitivity and localization within cells.
- The study's findings on the relationship between the chemical structure of RhoVRs and their membrane behavior offer valuable insights for designing fluorescent dyes that better detect voltage changes.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!